1ik3: Difference between revisions
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New page: left|200px<br /> <applet load="1ik3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ik3, resolution 2.0Å" /> '''LIPOXYGENASE-3 (SOYB... |
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== | ==LIPOXYGENASE-3 (SOYBEAN) COMPLEX WITH 13(S)-HYDROPEROXY-9(Z),11(E)-OCTADECADIENOIC ACID== | ||
Polyunsaturated fatty acid metabolism is governed primarily by two | <StructureSection load='1ik3' size='340' side='right'caption='[[1ik3]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1ik3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Glycine_max Glycine max]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1IK3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1IK3 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=11O:(TRANS-12,13-EPOXY)-11-HYDROXY-9(Z)-OCTADECENOIC+ACID'>11O</scene>, <scene name='pdbligand=13R:13(R)-HYDROPEROXY-9(Z),11(E)-OCTADECADIENOIC+ACID'>13R</scene>, <scene name='pdbligand=13S:13(S)-HYDROPEROXY-9(Z),11(E)-OCTADECADIENOIC+ACID'>13S</scene>, <scene name='pdbligand=9OH:(TRANS-12,13-EPOXY)-9-HYDROXY-10(E)-OCTADECENOIC+ACID'>9OH</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ik3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ik3 OCA], [https://pdbe.org/1ik3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ik3 RCSB], [https://www.ebi.ac.uk/pdbsum/1ik3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ik3 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/LOX3_SOYBN LOX3_SOYBN] Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids containing a cis,cis-1,4-pentadiene structure. | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ik/1ik3_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ik3 ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Polyunsaturated fatty acid metabolism is governed primarily by two enzymes, prostaglandin H synthase and lipoxygenase. The crystal structure of the metastable product-oxidized purple form of soybean lipoxygenase-3 was determined at 2.0 A resolution. The data reveal that the chromophore corresponds to an iron-peroxide complex, a potential intermediate in the catalyzed reaction. A significant alteration of the iron site accompanies the formation of the complex. The structure, the first for a fatty acid-lipoxygenase complex, also reveals an unexpected mode of binding, and identifies amino acid residues that may play significant roles in catalysis, regio- and stereoselectivity. | |||
Three-dimensional structure of a purple lipoxygenase.,Skrzypczak-Jankun E, Bross RA, Carroll RT, Dunham WR, Funk MO Jr J Am Chem Soc. 2001 Nov 7;123(44):10814-20. PMID:11686682<ref>PMID:11686682</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1ik3" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Glycine max]] | [[Category: Glycine max]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Jr | [[Category: Funk Jr MO]] | ||
[[Category: Skrzypczak-Jankun | [[Category: Skrzypczak-Jankun E]] | ||
Latest revision as of 09:37, 25 December 2024
LIPOXYGENASE-3 (SOYBEAN) COMPLEX WITH 13(S)-HYDROPEROXY-9(Z),11(E)-OCTADECADIENOIC ACID
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