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New page: left|200px<br /> <applet load="1c1e" size="450" color="white" frame="true" align="right" spinBox="true" caption="1c1e, resolution 1.9Å" /> '''CRYSTAL STRUCTURE OF...
 
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[[Image:1c1e.gif|left|200px]]<br />
<applet load="1c1e" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1c1e, resolution 1.9&Aring;" />
'''CRYSTAL STRUCTURE OF A DIELS-ALDERASE CATALYTIC ANTIBODY 1E9 IN COMPLEX WITH ITS HAPTEN'''<br />


==Overview==
==CRYSTAL STRUCTURE OF A DIELS-ALDERASE CATALYTIC ANTIBODY 1E9 IN COMPLEX WITH ITS HAPTEN==
The crystal structure of an efficient Diels-Alder antibody catalyst at 1.9, angstrom resolution reveals almost perfect shape complementarity with its, transition state analog. Comparison with highly related progesterone and, Diels-Alderase antibodies that arose from the same primordial germ line, template shows the relatively subtle mutational steps that were able to, evolve both structural complementarity and catalytic efficiency.
<StructureSection load='1c1e' size='340' side='right'caption='[[1c1e]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1c1e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. The September 2001 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Antibodies''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2001_9 10.2210/rcsb_pdb/mom_2001_9]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1C1E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1C1E FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ENH:1,7,8,9,10,10-HEXACHLORO-4-METHYL-4-AZA-TRICYCLO[5.2.1.0(2,6)]DEC-8-ENE-3,5-DIONE'>ENH</scene>, <scene name='pdbligand=MLT:D-MALATE'>MLT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1c1e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1c1e OCA], [https://pdbe.org/1c1e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1c1e RCSB], [https://www.ebi.ac.uk/pdbsum/1c1e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1c1e ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/c1/1c1e_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1c1e ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of an efficient Diels-Alder antibody catalyst at 1.9 angstrom resolution reveals almost perfect shape complementarity with its transition state analog. Comparison with highly related progesterone and Diels-Alderase antibodies that arose from the same primordial germ line template shows the relatively subtle mutational steps that were able to evolve both structural complementarity and catalytic efficiency.


==About this Structure==
Evolution of shape complementarity and catalytic efficiency from a primordial antibody template.,Xu J, Deng Q, Chen J, Houk KN, Bartek J, Hilvert D, Wilson IA Science. 1999 Dec 17;286(5448):2345-8. PMID:10600746<ref>PMID:10600746</ref>
1C1E is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ENH and DMR as [http://en.wikipedia.org/wiki/ligands ligands]. The following page contains interesting information on the relation of 1C1E with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb21_1.html Antibodies]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1C1E OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Evolution of shape complementarity and catalytic efficiency from a primordial antibody template., Xu J, Deng Q, Chen J, Houk KN, Bartek J, Hilvert D, Wilson IA, Science. 1999 Dec 17;286(5448):2345-8. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10600746 10600746]
</div>
<div class="pdbe-citations 1c1e" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Antibodies]]
[[Category: Antibodies]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Wilson, I.A.]]
[[Category: Wilson IA]]
[[Category: Xu, J.]]
[[Category: Xu J]]
[[Category: DMR]]
[[Category: ENH]]
[[Category: catalytic antibody]]
[[Category: diels-alder]]
[[Category: immunoglobulin]]
 
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Retrieved from "https://proteopedia.org/1c1e"