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New page: left|200px<br /> <applet load="1cgp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cgp, resolution 3.000Å" /> '''CATABOLITE GENE AC...
 
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[[Image:1cgp.gif|left|200px]]<br />
<applet load="1cgp" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1cgp, resolution 3.000&Aring;" />
'''CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE'''<br />


==Overview==
==CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE==
The 3 angstrom resolution crystal structure of the Escherichia coli, catabolite gene activator protein (CAP) complexed with a 30-base pair DNA, sequence shows that the DNA is bent by 90 degrees. This bend results, almost entirely from two 40 degrees kinks that occur between TG/CA base, pairs at positions 5 and 6 on each side of the dyad axis of the complex., DNA sequence discrimination by CAP derives both from sequence-dependent, distortion of the DNA helix and from direct hydrogen-bonding interactions, between three protein side chains and the exposed edges of three base, pairs in the major groove of the DNA. The structure of this transcription, factor--DNA complex provides insights into possible mechanisms of, transcription activation.
<StructureSection load='1cgp' size='340' side='right'caption='[[1cgp]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1cgp]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. The December 2003 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Catabolite Activator Protein''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2003_12 10.2210/rcsb_pdb/mom_2003_12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CGP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CGP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CMP:ADENOSINE-3,5-CYCLIC-MONOPHOSPHATE'>CMP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cgp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cgp OCA], [https://pdbe.org/1cgp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cgp RCSB], [https://www.ebi.ac.uk/pdbsum/1cgp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cgp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CRP_ECOLI CRP_ECOLI] This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of several catabolite-sensitive operons. The protein induces a severe bend in the DNA. Acts as a negative regulator of its own synthesis as well as for adenylate cyclase (cyaA), which generates cAMP.<ref>PMID:2982847</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cg/1cgp_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cgp ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1CGP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with CMP as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1CGP with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb48_1.html Catabolite Activator Protein]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CGP OCA].
*[[Catabolite gene activator protein 3D structures|Catabolite gene activator protein 3D structures]]
 
== References ==
==Reference==
<references/>
Crystal structure of a CAP-DNA complex: the DNA is bent by 90 degrees., Schultz SC, Shields GC, Steitz TA, Science. 1991 Aug 30;253(5023):1001-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1653449 1653449]
__TOC__
</StructureSection>
[[Category: Catabolite Activator Protein]]
[[Category: Catabolite Activator Protein]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Schultz, S.C.]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Shields, G.C]]
[[Category: Synthetic construct]]
[[Category: Steitz, T.A.]]
[[Category: Schultz SC]]
[[Category: CMP]]
[[Category: Shields GC]]
[[Category: double helix]]
[[Category: Steitz TA]]
[[Category: protein-dna complex]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 08:58:22 2007''

Latest revision as of 06:42, 7 February 2024

CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

1cgp, resolution 3.00Å

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