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[[Image:2o3d.gif|left|200px]]
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{{STRUCTURE_2o3d|  PDB=2o3d  |  SCENE=  }}
'''Structure of human SF2/ASF RNA recognition motif 2 (RRM2)'''


==Structure of human SF2/ASF RNA recognition motif 2 (RRM2)==
<StructureSection load='2o3d' size='340' side='right'caption='[[2o3d]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2o3d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O3D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O3D FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o3d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o3d OCA], [https://pdbe.org/2o3d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o3d RCSB], [https://www.ebi.ac.uk/pdbsum/2o3d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o3d ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SRSF1_HUMAN SRSF1_HUMAN] Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors.<ref>PMID:8223481</ref> <ref>PMID:8139654</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o3/2o3d_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2o3d ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The serine/arginine-rich (SR) protein splicing factor 2/alternative splicing factor (SF2/ASF) has a role in splicing, stability, export and translation of messenger RNA. Here, we present the structure of the RNA recognition motif (RRM) 2 from SF2/ASF, which has an RRM fold with a considerably extended loop 5 region, containing a two-stranded beta-sheet. The loop 5 extension places the previously identified SR protein kinase 1 docking sequence largely within the RRM fold. We show that RRM2 binds to RNA in a new way, by using a tryptophan within a conserved SWQLKD motif that resides on helix alpha1, together with amino acids from strand beta2 and a histidine on loop 5. The linker connecting RRM1 and RRM2 contains arginine residues, which provide a binding site for the mRNA export factor TAP, and when TAP binds to this region it displaces RNA bound to RRM2.


==Overview==
Structural and functional analysis of RNA and TAP binding to SF2/ASF.,Tintaru AM, Hautbergue GM, Hounslow AM, Hung ML, Lian LY, Craven CJ, Wilson SA EMBO Rep. 2007 Aug;8(8):756-62. Epub 2007 Jul 13. PMID:17668007<ref>PMID:17668007</ref>
The serine/arginine-rich (SR) protein splicing factor 2/alternative splicing factor (SF2/ASF) has a role in splicing, stability, export and translation of messenger RNA. Here, we present the structure of the RNA recognition motif (RRM) 2 from SF2/ASF, which has an RRM fold with a considerably extended loop 5 region, containing a two-stranded beta-sheet. The loop 5 extension places the previously identified SR protein kinase 1 docking sequence largely within the RRM fold. We show that RRM2 binds to RNA in a new way, by using a tryptophan within a conserved SWQLKD motif that resides on helix alpha1, together with amino acids from strand beta2 and a histidine on loop 5. The linker connecting RRM1 and RRM2 contains arginine residues, which provide a binding site for the mRNA export factor TAP, and when TAP binds to this region it displaces RNA bound to RRM2.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2O3D is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O3D OCA].
</div>
<div class="pdbe-citations 2o3d" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structural and functional analysis of RNA and TAP binding to SF2/ASF., Tintaru AM, Hautbergue GM, Hounslow AM, Hung ML, Lian LY, Craven CJ, Wilson SA, EMBO Rep. 2007 Aug;8(8):756-62. Epub 2007 Jul 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17668007 17668007]
*[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Craven, C J.]]
[[Category: Craven CJ]]
[[Category: Hautbergue, G M.]]
[[Category: Hautbergue GM]]
[[Category: Hounslow, A M.]]
[[Category: Hounslow AM]]
[[Category: Lian, L Y.]]
[[Category: Lian LY]]
[[Category: Tintaru, A M.]]
[[Category: Tintaru AM]]
[[Category: Wilson, S A.]]
[[Category: Wilson SA]]
[[Category: Rna binding protein]]
[[Category: Rrm domain]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 10:16:36 2008''
Retrieved from "https://proteopedia.org/2o3d"