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[[Image:2ofm.jpg|left|200px]]
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{{STRUCTURE_2ofm|  PDB=2ofm  |  SCENE=  }}
'''1.11 A Crystal Structure of Apo Nitrophorin 4 From Rhodnius Prolixus'''


==1.11 A Crystal Structure of Apo Nitrophorin 4 From Rhodnius Prolixus==
<StructureSection load='2ofm' size='340' side='right'caption='[[2ofm]], [[Resolution|resolution]] 1.11&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2ofm]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2OFM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.11&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ofm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ofm OCA], [https://pdbe.org/2ofm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ofm RCSB], [https://www.ebi.ac.uk/pdbsum/2ofm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ofm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/NP4_RHOPR NP4_RHOPR] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/2ofm_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ofm ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The nitrophorins from Rhodnius prolixus, the kissing bug, are heme-containing proteins used for the transport of nitric oxide to aide the insect in obtaining a blood meal. The Rhodnius nitrophorins display an eight-stranded antiparallel beta-barrel motif, typical of lipocalins, with a histidine-linked heme in the open end of the barrel. Heme is stabilized in the ferric state and highly distorted, displaying a ruffled conformation that may be of importance in the setting of the reduction potential. To help in understanding the means by which the protein matrix, an inherently soft material, is able to distort the heme from its low-energy planar conformation, we have determined the crystal structure of apo-nitrophorin 4-1.1 A resolution. Removal of the heme from nitrophorin 4 has very little effect on its structure: The heme binding cavity remains open and the loops near the cavity entrance respond to lower pH in the same manner as the intact protein. We conclude that the general stability of the lipocalin fold and apparent rigidity of the beta-barrel provide the means for distorting the heme cofactor.


==Overview==
Apo-nitrophorin 4 at atomic resolution.,Amoia AM, Montfort WR Protein Sci. 2007 Sep;16(9):2076-81. Epub 2007 Jul 27. PMID:17660249<ref>PMID:17660249</ref>
The nitrophorins from Rhodnius prolixus, the kissing bug, are heme-containing proteins used for the transport of nitric oxide to aide the insect in obtaining a blood meal. The Rhodnius nitrophorins display an eight-stranded antiparallel beta-barrel motif, typical of lipocalins, with a histidine-linked heme in the open end of the barrel. Heme is stabilized in the ferric state and highly distorted, displaying a ruffled conformation that may be of importance in the setting of the reduction potential. To help in understanding the means by which the protein matrix, an inherently soft material, is able to distort the heme from its low-energy planar conformation, we have determined the crystal structure of apo-nitrophorin 4-1.1 A resolution. Removal of the heme from nitrophorin 4 has very little effect on its structure: The heme binding cavity remains open and the loops near the cavity entrance respond to lower pH in the same manner as the intact protein. We conclude that the general stability of the lipocalin fold and apparent rigidity of the beta-barrel provide the means for distorting the heme cofactor.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2OFM is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2OFM OCA].
</div>
<div class="pdbe-citations 2ofm" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Apo-nitrophorin 4 at atomic resolution., Amoia AM, Montfort WR, Protein Sci. 2007 Sep;16(9):2076-81. Epub 2007 Jul 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17660249 17660249]
*[[Nitrophorin|Nitrophorin]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Rhodnius prolixus]]
[[Category: Rhodnius prolixus]]
[[Category: Single protein]]
[[Category: Amoia AM]]
[[Category: Amoia, A M.]]
[[Category: Absent cofactor]]
[[Category: Beta barrel]]
[[Category: Lipocalin]]
[[Category: Transport protein]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 10:48:30 2008''

Latest revision as of 10:37, 30 August 2023

1.11 A Crystal Structure of Apo Nitrophorin 4 From Rhodnius Prolixus

2ofm, resolution 1.11Å

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