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New page: left|200px<br /> <applet load="1yyf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yyf, resolution 4.16Å" /> '''Correction of X-ray...
 
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[[Image:1yyf.gif|left|200px]]<br />
<applet load="1yyf" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1yyf, resolution 4.16&Aring;" />
'''Correction of X-ray Intensities from an HslV-HslU co-crystal containing lattice translocation defects'''<br />


==Overview==
==Correction of X-ray Intensities from an HslV-HslU co-crystal containing lattice translocation defects==
Because of lattice-translocation defects, two identical but translated, lattices can coexist as a single coherent mosaic block in a crystal. The, observed structure in such cases is a weighted sum of two identical but, translated structures, one from each lattice; the observed structure, factors are a weighted vector sum of the structure factors with identical, unit amplitudes but shifted phases. The correction of X-ray intensities, from a single crystal containing these defects of the hybrid HslV-HslU, complex, which consists of Escherichia coli HslU and Bacillus subtilis, HslV (also known as CodW), is reported. When intensities are not, corrected, a biologically irrelevant complex (with CodW from one lattice, and HslU from another) is implied to exist. Only upon correction does a, biologically functional CodW-HslU complex structure emerge.
<StructureSection load='1yyf' size='340' side='right'caption='[[1yyf]], [[Resolution|resolution]] 4.16&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1yyf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. The August 2006 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''AAA+ Proteases''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2006_8 10.2210/rcsb_pdb/mom_2006_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YYF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YYF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.16&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yyf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yyf OCA], [https://pdbe.org/1yyf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yyf RCSB], [https://www.ebi.ac.uk/pdbsum/1yyf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yyf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HSLU_ECOLI HSLU_ECOLI] ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N-terminal part of its protein substrates and unfolds these before they are guided to HslV for hydrolysis.<ref>PMID:8662828</ref> <ref>PMID:8650174</ref> <ref>PMID:9288941</ref> <ref>PMID:9393683</ref> <ref>PMID:10452560</ref> <ref>PMID:10419524</ref> <ref>PMID:15696175</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/yy/1yyf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1yyf ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1YYF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis] and [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ADP as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1YYF with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb80_1.html AAA+ Proteases]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1YYF OCA].
*[[Heat Shock Protein structures|Heat Shock Protein structures]]
 
== References ==
==Reference==
<references/>
Correction of X-ray intensities from an HslV-HslU co-crystal containing lattice-translocation defects., Wang J, Rho SH, Park HH, Eom SH, Acta Crystallogr D Biol Crystallogr. 2005 Jul;61(Pt 7):932-41. Epub 2005, Jun 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15983416 15983416]
__TOC__
</StructureSection>
[[Category: AAA+ Proteases]]
[[Category: AAA+ Proteases]]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Eom, S.H.]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Park, H.H.]]
[[Category: Eom SH]]
[[Category: Rho, S.H.]]
[[Category: Park HH]]
[[Category: Wang, J.]]
[[Category: Rho SH]]
[[Category: ADP]]
[[Category: Wang J]]
[[Category: atp-dependent proteolysis]]
[[Category: hslv-hslu]]
[[Category: lattice translocation defect]]
[[Category: quaternary structure]]
 
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Retrieved from "https://proteopedia.org/1yyf"