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New page: left|200px<br /> <applet load="1zaa" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zaa, resolution 2.100Å" /> '''ZINC FINGER-DNA RE...
 
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[[Image:1zaa.gif|left|200px]]<br />
<applet load="1zaa" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1zaa, resolution 2.100&Aring;" />
'''ZINC FINGER-DNA RECOGNITION: CRYSTAL STRUCTURE OF A ZIF268-DNA COMPLEX AT 2.1 ANGSTROMS'''<br />


==Overview==
==ZINC FINGER-DNA RECOGNITION: CRYSTAL STRUCTURE OF A ZIF268-DNA COMPLEX AT 2.1 ANGSTROMS==
The zinc finger DNA-binding motif occurs in many proteins that regulate, eukaryotic gene expression. The crystal structure of a complex containing, the three zinc fingers from Zif268 (a mouse immediate early protein) and a, consensus DNA-binding site has been determined at 2.1 angstroms resolution, and refined to a crystallographic R factor of 18.2 percent. In this, complex, the zinc fingers bind in the major groove of B-DNA and wrap part, way around the double helix. Each finger has a similar relation to the DNA, and makes its primary contacts in a three-base pair subsite. Residues from, the amino-terminal portion of an alpha helix contact the bases, and most, of the contracts are made with the guanine-rich strand of the DNA. This, structure provides a framework for understanding how zinc fingers, recognize DNA and suggests that this motif may provide a useful basis for, the design of novel DNA-binding proteins.
<StructureSection load='1zaa' size='340' side='right'caption='[[1zaa]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1zaa]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. The March 2007 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Zinc Fingers''  by David S. Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2007_3 10.2210/rcsb_pdb/mom_2007_3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZAA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZAA FirstGlance]. <br>
1ZAA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. The following page contains interesting information on the relation of 1ZAA with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb87_1.html Zinc Fingers]]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZAA OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zaa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zaa OCA], [https://pdbe.org/1zaa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zaa RCSB], [https://www.ebi.ac.uk/pdbsum/1zaa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zaa ProSAT]</span></td></tr>
Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A., Pavletich NP, Pabo CO, Science. 1991 May 10;252(5007):809-17. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2028256 2028256]
</table>
== Function ==
[https://www.uniprot.org/uniprot/EGR1_MOUSE EGR1_MOUSE] Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/za/1zaa_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1zaa ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Zinc Fingers]]
[[Category: Zinc Fingers]]
[[Category: Pabo, C.O.]]
[[Category: Pabo CO]]
[[Category: Pavletich, N.P.]]
[[Category: Pavletich NP]]
[[Category: ZN]]
[[Category: double helix]]
[[Category: protein-dna complex]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:07:39 2007''

Latest revision as of 09:03, 14 February 2024

ZINC FINGER-DNA RECOGNITION: CRYSTAL STRUCTURE OF A ZIF268-DNA COMPLEX AT 2.1 ANGSTROMS

1zaa, resolution 2.10Å

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