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| | ==Crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1== |
| The line below this paragraph, containing "STRUCTURE_2p6w", creates the "Structure Box" on the page.
| | <StructureSection load='2p6w' size='340' side='right'caption='[[2p6w]], [[Resolution|resolution]] 1.60Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[2p6w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paramecium_bursaria_Chlorella_virus_1 Paramecium bursaria Chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2P6W FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
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| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr> |
| {{STRUCTURE_2p6w| PDB=2p6w | SCENE= }}
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2p6w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p6w OCA], [https://pdbe.org/2p6w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2p6w RCSB], [https://www.ebi.ac.uk/pdbsum/2p6w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2p6w ProSAT]</span></td></tr> |
| | | </table> |
| '''Crystal structure of a glycosyltransferase involved in the glycosylation of the major capsid of PBCV-1'''
| | == Function == |
| | | [https://www.uniprot.org/uniprot/Q89399_PBCV1 Q89399_PBCV1] |
| | | __TOC__ |
| ==Overview== | | </StructureSection> |
| Paramecium bursaria chlorella virus-1 encodes at least five putative glycosyltransferases that are probably involved in the synthesis of the glycan components of the viral major capsid protein. The 1.6 A crystal structure of one of these glycosyltransferases (A64R) has a mixed alpha/beta fold containing a central, six-stranded beta sheet flanked by alpha helices. Crystal structures of A64R, complexed with UDP, CMP, or GDP, established that only UDP bound to A64R in the presence of Mn(2+), consistent with its high structural similarity to glycosyltransferases which utilize UDP as the sugar carrier. The structure of the complex of A64R, UDP-glucose, and Mn(2+) showed that the largest conformational change occurred when hydrogen bonds were formed with the ligands. Unlike UDP-glucose, UDP-galactose and UDP-GlcNAc did not bind to A64R, suggesting a selective binding of UDP-glucose. Thus, UDP-glucose is most likely the sugar donor for A64R, consistent with glucose occurring in the virus major capsid protein glycans.
| | [[Category: Large Structures]] |
| | | [[Category: Paramecium bursaria Chlorella virus 1]] |
| ==About this Structure== | | [[Category: Rossmann MG]] |
| 2P6W is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Paramecium_bursaria_chlorella_virus_1 Paramecium bursaria chlorella virus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2P6W OCA].
| | [[Category: Van Etten JL]] |
| | | [[Category: Ye X]] |
| ==Reference== | | [[Category: Zhang Y]] |
| Structure and function of a chlorella virus-encoded glycosyltransferase., Zhang Y, Xiang Y, Van Etten JL, Rossmann MG, Structure. 2007 Sep;15(9):1031-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17850743 17850743]
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| [[Category: Paramecium bursaria chlorella virus 1]] | |
| [[Category: Single protein]] | |
| [[Category: Etten, J L.Van.]] | |
| [[Category: Rossmann, M G.]] | |
| [[Category: Ye, X.]] | |
| [[Category: Zhang, Y.]] | |
| [[Category: Crystal structure]] | |
| [[Category: Glycosyltransferase]] | |
| [[Category: Pbcv]] | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 12:30:35 2008''
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