1i3u: Difference between revisions

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New page: left|200px<br /> <applet load="1i3u" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i3u, resolution 1.95Å" /> '''THREE-DIMENSIONAL S...
 
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[[Image:1i3u.gif|left|200px]]<br />
<applet load="1i3u" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1i3u, resolution 1.95&Aring;" />
'''THREE-DIMENSIONAL STRUCTURE OF A LLAMA VHH DOMAIN COMPLEXED WITH THE DYE RR1'''<br />


==Overview==
==THREE-DIMENSIONAL STRUCTURE OF A LLAMA VHH DOMAIN COMPLEXED WITH THE DYE RR1==
Camelids, camels and llamas, have a unique immune system able to produce, heavy-chain only antibodies. Their VH domains (VHHs) are the smallest, binding units produced by immune systems, and therefore suitable for, biotechnological applications through heterologous expression. The, recognition of protein antigens by these VHHs is rather well documented, while less is known about the VHH/hapten interactions. The recently, reported X-ray structure of a VHH in complex with a copper-containing, azo-dye settled the ability of VHH to recognize haptens by forming a, cavity between the three complementarity-determining regions (CDR). Here, we report the structures of a VHH (VHH A52) free or complexed with an, azo-dye, RR1, without metal ion. The structure of the complex illustrates, the involvement of CDR2, CDR3 and a framework residue in a lateral, interaction with the hapten. Such a lateral combining site is comparable, to that found in classical antibodies, although in the absence of the VL.
<StructureSection load='1i3u' size='340' side='right'caption='[[1i3u]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1i3u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I3U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I3U FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CGN:5-OXO-PYRROLIDINE-2-CARBALDEHYDE'>CGN</scene>, <scene name='pdbligand=RR1:5-(4,6-DIAMINO-[1,3,5]TRIAZIN-2-YLAMINO)-4-HYDROXY-3-(2-SULFO-PHENYLAZO)-NAPHTHALENE-2,7-DISULFONIC+ACID'>RR1</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i3u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i3u OCA], [https://pdbe.org/1i3u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i3u RCSB], [https://www.ebi.ac.uk/pdbsum/1i3u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i3u ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i3/1i3u_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i3u ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1I3U is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Lama_glama Lama glama] with SO4 and RR1 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I3U OCA].
*[[Antibody 3D structures|Antibody 3D structures]]
 
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
==Reference==
__TOC__
Lateral recognition of a dye hapten by a llama VHH domain., Spinelli S, Tegoni M, Frenken L, van Vliet C, Cambillau C, J Mol Biol. 2001 Aug 3;311(1):123-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11469862 11469862]
</StructureSection>
[[Category: Lama glama]]
[[Category: Lama glama]]
[[Category: Protein complex]]
[[Category: Large Structures]]
[[Category: Cambillau, C.]]
[[Category: Cambillau C]]
[[Category: Frenken, L.]]
[[Category: Frenken L]]
[[Category: Spinelli, S.]]
[[Category: Spinelli S]]
[[Category: Tegoni, M.]]
[[Category: Tegoni M]]
[[Category: Vliet, C.van.]]
[[Category: Van Vliet C]]
[[Category: RR1]]
[[Category: SO4]]
[[Category: antibody]]
[[Category: lama glama]]
[[Category: vhh fragment]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:32:23 2007''

Latest revision as of 06:01, 3 April 2024

THREE-DIMENSIONAL STRUCTURE OF A LLAMA VHH DOMAIN COMPLEXED WITH THE DYE RR1

1i3u, resolution 1.95Å

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