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New page: left|200px<br /> <applet load="1i8k" size="450" color="white" frame="true" align="right" spinBox="true" caption="1i8k, resolution 1.80Å" /> '''CRYSTAL STRUCTURE O...
 
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[[Image:1i8k.gif|left|200px]]<br />
<applet load="1i8k" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1i8k, resolution 1.80&Aring;" />
'''CRYSTAL STRUCTURE OF DSFV MR1 IN COMPLEX WITH THE PEPTIDE ANTIGEN OF THE MUTANT EPIDERMAL GROWTH FACTOR RECEPTOR, EGFRVIII, AT LIQUID NITROGEN TEMPERATURE'''<br />


==Overview==
==CRYSTAL STRUCTURE OF DSFV MR1 IN COMPLEX WITH THE PEPTIDE ANTIGEN OF THE MUTANT EPIDERMAL GROWTH FACTOR RECEPTOR, EGFRVIII, AT LIQUID NITROGEN TEMPERATURE==
Epitope mapping studies and the determination of the structure to 1.8 A, resolution have been carried out for the antigen-binding fragment MR1 in, complex with peptide antigen. MR1 is specific for the novel fusion, junction of the mutant epidermal growth factor receptor EGFRvIII and has, been reported to have a high degree of specificity for the mutant EGFRvIII, over the wild-type EGF receptor. The structure of the complex shows that, the peptide antigen residue side-chains found by epitope mapping studies, to be critical for recognition are accommodated in pockets on the surface, of the Fv. However, the most distinctive portion of the peptide antigen, the novel fusion glycine residue, makes no contact to the Fv and does not, contribute directly to the epitope. The specificity of MR1 lies in the, ability of this glycine residue to assume the restricted conformation, needed to form a type II' beta-hairpin turn more easily, and demonstrates, that a peptide antigen can be used to generate a conformational epitope.
<StructureSection load='1i8k' size='340' side='right'caption='[[1i8k]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1i8k]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1I8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1I8K FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1i8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1i8k OCA], [https://pdbe.org/1i8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1i8k RCSB], [https://www.ebi.ac.uk/pdbsum/1i8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1i8k ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/KV3A8_MOUSE KV3A8_MOUSE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/i8/1i8k_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1i8k ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1I8K is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1I8K OCA].
*[[Antibody 3D structures|Antibody 3D structures]]
 
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
==Reference==
__TOC__
Antibody recognition of a conformational epitope in a peptide antigen: Fv-peptide complex of an antibody fragment specific for the mutant EGF receptor, EGFRvIII., Landry RC, Klimowicz AC, Lavictoire SJ, Borisova S, Kottachchi DT, Lorimer IA, Evans SV, J Mol Biol. 2001 May 18;308(5):883-93. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11352579 11352579]
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Protein complex]]
[[Category: Borisova S]]
[[Category: Borisova, S.]]
[[Category: Evans SV]]
[[Category: Evans, S.V.]]
[[Category: Klimowicz AC]]
[[Category: Klimowicz, A.C.]]
[[Category: Kottachchi DT]]
[[Category: Kottachchi, D.T.]]
[[Category: Landry RC]]
[[Category: Landry, R.C.]]
[[Category: Lavictoire SJ]]
[[Category: Lavictoire, S.J.]]
[[Category: Lorimer IA]]
[[Category: Lorimer, I.A.]]
[[Category: antibody-peptide complex]]
[[Category: immunoglobulin fold]]
[[Category: peptide antigen]]
[[Category: type ii' beta turn.]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:32:32 2007''

Latest revision as of 07:45, 3 April 2024

CRYSTAL STRUCTURE OF DSFV MR1 IN COMPLEX WITH THE PEPTIDE ANTIGEN OF THE MUTANT EPIDERMAL GROWTH FACTOR RECEPTOR, EGFRVIII, AT LIQUID NITROGEN TEMPERATURE

1i8k, resolution 1.80Å

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