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[[Image:2pff.jpg|left|200px]]
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{{STRUCTURE_2pff|  PDB=2pff  |  SCENE=  }}
'''Structural Insights of Yeast Fatty Acid Synthase'''


==Structural Insights of Yeast Fatty Acid Synthase==
<StructureSection load='2pff' size='340' side='right'caption='[[2pff]], [[Resolution|resolution]] 4.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2pff]] is a 9 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2PFF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2pff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2pff OCA], [https://pdbe.org/2pff PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2pff RCSB], [https://www.ebi.ac.uk/pdbsum/2pff PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2pff ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FAS2_YEAST FAS2_YEAST] Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The alpha subunit contains domains for: acyl carrier protein, 3-oxoacyl-[acyl-carrier-protein] reductase, and 3-oxoacyl-[acyl-carrier-protein] synthase. This subunit coordinates the binding of the six beta subunits to the enzyme complex.[HAMAP-Rule:MF_00101]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In yeast, the whole metabolic pathway for making 16- and 18-carbon fatty acids is carried out by fatty acid synthase, a 2.6 megadalton molecular-weight macromolecular assembly containing six copies of all eight catalytic centers. We have determined its crystal structure, which illuminates how this enzyme is initially activated and then carries out multiple steps of synthesis in each of six sterically isolated reaction chambers. Six of the catalytic sites are in the wall of the assembly facing an acyl carrier protein (ACP) bound to the ketoacyl synthase domain. Two-dimensional diffusion of substrates to the catalytic sites may be achieved by the electrostatically negative ACP swinging to each of the six electrostatically positive catalytic sites. The phosphopantetheinyl transferase domain lies outside the shell of the assembly, inaccessible to ACP that lies inside, suggesting that the attachment of the pantetheine arm to ACP must occur before complete assembly of the complex.


==Overview==
The crystal structure of yeast fatty acid synthase, a cellular machine with eight active sites working together.,Lomakin IB, Xiong Y, Steitz TA Cell. 2007 Apr 20;129(2):319-32. PMID:17448991<ref>PMID:17448991</ref>
In yeast, the whole metabolic pathway for making 16- and 18-carbon fatty acids is carried out by fatty acid synthase, a 2.6 megadalton molecular-weight macromolecular assembly containing six copies of all eight catalytic centers. We have determined its crystal structure, which illuminates how this enzyme is initially activated and then carries out multiple steps of synthesis in each of six sterically isolated reaction chambers. Six of the catalytic sites are in the wall of the assembly facing an acyl carrier protein (ACP) bound to the ketoacyl synthase domain. Two-dimensional diffusion of substrates to the catalytic sites may be achieved by the electrostatically negative ACP swinging to each of the six electrostatically positive catalytic sites. The phosphopantetheinyl transferase domain lies outside the shell of the assembly, inaccessible to ACP that lies inside, suggesting that the attachment of the pantetheine arm to ACP must occur before complete assembly of the complex.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2PFF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2PFF OCA].
</div>
<div class="pdbe-citations 2pff" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
The crystal structure of yeast fatty acid synthase, a cellular machine with eight active sites working together., Lomakin IB, Xiong Y, Steitz TA, Cell. 2007 Apr 20;129(2):319-32. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17448991 17448991]
*[[Fatty acid synthase 3D structures|Fatty acid synthase 3D structures]]
[[Category: Fatty-acyl-CoA synthase]]
== References ==
[[Category: Protein complex]]
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Lomakin, I B.]]
[[Category: Lomakin IB]]
[[Category: Steitz, T A.]]
[[Category: Steitz TA]]
[[Category: Xiong, Y.]]
[[Category: Xiong Y]]
[[Category: Acetyltransferase]]
[[Category: Acyl-carrier-protein]]
[[Category: Beta-ketoacyl reductase]]
[[Category: Beta-ketoacyl synthase]]
[[Category: Dehydratase]]
[[Category: Fatty acid synthase]]
[[Category: Malonyltransferase]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 13:00:10 2008''
Retrieved from "https://proteopedia.org/2pff"