1ngx: Difference between revisions

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New page: left|200px<br /> <applet load="1ngx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ngx, resolution 1.80Å" /> '''Chimeric Germline F...
 
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[[Image:1ngx.gif|left|200px]]<br />
<applet load="1ngx" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ngx, resolution 1.80&Aring;" />
'''Chimeric Germline Fab 7g12 with jeffamine fragment bound'''<br />


==Overview==
==Chimeric Germline Fab 7g12 with jeffamine fragment bound==
The crystal structure of the Michaelis complex between the Fab fragment of, ferrochelatase antibody 7G12 and its substrate mesoporphyrin has been, solved to 2.6-A resolution. The antibody-bound mesoporphyrin clearly, adopts a nonplanar conformation and reveals that the antibody catalyzes, the porphyrin metallation reaction by straining/distorting the bound, substrate toward the transition-state configuration. The crystal, structures of the Fab fragment of the germ-line precursor antibody to 7G12, and its complex with the hapten N-methylmesoporphyrin have also been, solved. A comparison of these structures with the corresponding structures, of the affinity-matured antibody 7G12 reveals the molecular mechanism by, which the immune system evolves binding energy to catalyze this reaction.
<StructureSection load='1ngx' size='340' side='right'caption='[[1ngx]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ngx]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NGX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NGX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=JEF:O-(O-(2-AMINOPROPYL)-O-(2-METHOXYETHYL)POLYPROPYLENE+GLYCOL+500)'>JEF</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ngx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ngx OCA], [https://pdbe.org/1ngx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ngx RCSB], [https://www.ebi.ac.uk/pdbsum/1ngx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ngx ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ng/1ngx_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ngx ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1NGX is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with JEF as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1NGX OCA].
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Structural evidence for substrate strain in antibody catalysis., Yin J, Andryski SE, Beuscher AE 4th, Stevens RC, Schultz PG, Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):856-61. Epub 2003 Jan 24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12552112 12552112]
[[Category: Homo sapiens]]
[[Category: ]]
[[Category: Large Structures]]
[[Category: Protein complex]]
[[Category: Mus musculus]]
[[Category: Andryski, S.E.]]
[[Category: Andryski SE]]
[[Category: Beuscher, A.B.]]
[[Category: Beuscher AB]]
[[Category: Schultz, P.G.]]
[[Category: Schultz PG]]
[[Category: Stevens, R.C.]]
[[Category: Stevens RC]]
[[Category: Yin, J.]]
[[Category: Yin J]]
[[Category: JEF]]
[[Category: antibody]]
[[Category: antigen binding fragment (fab)]]
[[Category: immunoglobulin]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:38:29 2007''

Latest revision as of 08:49, 10 April 2024

Chimeric Germline Fab 7g12 with jeffamine fragment bound

1ngx, resolution 1.80Å

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