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[[Image:2q2l.jpg|left|200px]]
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{{STRUCTURE_2q2l|  PDB=2q2l  |  SCENE=  }}
'''Crystal Structure of Superoxide Dismutase from P. atrosanguina'''


==Crystal Structure of Superoxide Dismutase from P. atrosanguina==
<StructureSection load='2q2l' size='340' side='right'caption='[[2q2l]], [[Resolution|resolution]] 2.37&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2q2l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Potentilla_atrosanguinea Potentilla atrosanguinea]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q2L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Q2L FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.367&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2q2l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2q2l OCA], [https://pdbe.org/2q2l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2q2l RCSB], [https://www.ebi.ac.uk/pdbsum/2q2l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2q2l ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B2CP37_9ROSA B2CP37_9ROSA] Destroys radicals which are normally produced within the cells and which are toxic to biological systems.[RuleBase:RU000393]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/q2/2q2l_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2q2l ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Superoxide dismutase (SOD) from Potentilla atrosanguinea (Wall. ex. Lehm.) was crystallized using 20% PEG 3350 and 0.2 M ammonium iodide and diffraction data were collected to 2.36 A resolution using an in-house Cu Kalpha X-ray source. Analyses show that data with a redundancy of 3.2 were sufficient to determine the structure by the SAD technique using the iodine anomalous signal. This redundancy is lower than that in previous cases in which protein structures were determined using iodines for phasing and in-house copper X-ray sources. Cocrystallization of proteins with halide salts such as ammonium iodide in combination with copper-anode X-ray radiation can therefore serve as a powerful and easy avenue for structure solution.


==Overview==
SAD phasing of a structure based on cocrystallized iodides using an in-house Cu Kalpha X-ray source: effects of data redundancy and completeness on structure solution.,Yogavel M, Gill J, Mishra PC, Sharma A Acta Crystallogr D Biol Crystallogr. 2007 Aug;63(Pt 8):931-4. Epub 2007, Jul 17. PMID:17642520<ref>PMID:17642520</ref>
Superoxide dismutase (SOD) from Potentilla atrosanguinea (Wall. ex. Lehm.) was crystallized using 20% PEG 3350 and 0.2 M ammonium iodide and diffraction data were collected to 2.36 A resolution using an in-house Cu Kalpha X-ray source. Analyses show that data with a redundancy of 3.2 were sufficient to determine the structure by the SAD technique using the iodine anomalous signal. This redundancy is lower than that in previous cases in which protein structures were determined using iodines for phasing and in-house copper X-ray sources. Cocrystallization of proteins with halide salts such as ammonium iodide in combination with copper-anode X-ray radiation can therefore serve as a powerful and easy avenue for structure solution.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Q2L OCA].
</div>
<div class="pdbe-citations 2q2l" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
SAD phasing of a structure based on cocrystallized iodides using an in-house Cu Kalpha X-ray source: effects of data redundancy and completeness on structure solution., Yogavel M, Gill J, Mishra PC, Sharma A, Acta Crystallogr D Biol Crystallogr. 2007 Aug;63(Pt 8):931-4. Epub 2007, Jul 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17642520 17642520]
*[[Superoxide dismutase 3D structures|Superoxide dismutase 3D structures]]
[[Category: Superoxide dismutase]]
== References ==
[[Category: Gill, J.]]
<references/>
[[Category: Manickam, Y.]]
__TOC__
[[Category: Mishra, P C.]]
</StructureSection>
[[Category: Sharma, A.]]
[[Category: Large Structures]]
[[Category: Antioxidant]]
[[Category: Potentilla atrosanguinea]]
[[Category: Metal-binding]]
[[Category: Gill J]]
[[Category: Oxidoreductase]]
[[Category: Manickam Y]]
[[Category: Sad]]
[[Category: Mishra PC]]
[[Category: Sod]]
[[Category: Sharma A]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 14:12:51 2008''

Latest revision as of 08:31, 30 October 2024

Crystal Structure of Superoxide Dismutase from P. atrosanguina

2q2l, resolution 2.37Å

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