2qe2: Difference between revisions

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[[Image:2qe2.jpg|left|200px]]
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{{STRUCTURE_2qe2|  PDB=2qe2  |  SCENE=  }}
'''Structure of HCV NS5B Bound to an Anthranilic Acid Inhibitor'''


==Structure of HCV NS5B Bound to an Anthranilic Acid Inhibitor==
<StructureSection load='2qe2' size='340' side='right'caption='[[2qe2]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2qe2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Hepatitis_C_virus_subtype_1b Hepatitis C virus subtype 1b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QE2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QE2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=452:2-{[N-(2-ACETYL-5-CHLORO-4-FLUOROPHENYL)GLYCYL]AMINO}BENZOIC+ACID'>452</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qe2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qe2 OCA], [https://pdbe.org/2qe2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qe2 RCSB], [https://www.ebi.ac.uk/pdbsum/2qe2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qe2 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q99AU2_9HEPC Q99AU2_9HEPC]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qe/2qe2_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qe2 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A series of potent anthranilic acid-based inhibitors of the hepatitis C NS5B polymerase has been identified. The inhibitors bind to a site on NS5B between the thumb and palm regions adjacent to the active site as determined by X-ray crystallography of the enzyme-inhibitor complex. Guided by both molecular modeling and traditional SAR, the enzyme activity of the initial hit was improved by approximately 100-fold, yielding a series of potent and selective NS5B inhibitors with IC50 values as low as 10 nM. These compounds were also inhibitors of the HCV replicon in cultured HUH7 cells.


==Overview==
Identification of anthranilic acid derivatives as a novel class of allosteric inhibitors of hepatitis C NS5B polymerase.,Nittoli T, Curran K, Insaf S, DiGrandi M, Orlowski M, Chopra R, Agarwal A, Howe AY, Prashad A, Floyd MB, Johnson B, Sutherland A, Wheless K, Feld B, O'Connell J, Mansour TS, Bloom J J Med Chem. 2007 May 3;50(9):2108-16. Epub 2007 Apr 3. PMID:17402724<ref>PMID:17402724</ref>
A series of potent anthranilic acid-based inhibitors of the hepatitis C NS5B polymerase has been identified. The inhibitors bind to a site on NS5B between the thumb and palm regions adjacent to the active site as determined by X-ray crystallography of the enzyme-inhibitor complex. Guided by both molecular modeling and traditional SAR, the enzyme activity of the initial hit was improved by approximately 100-fold, yielding a series of potent and selective NS5B inhibitors with IC50 values as low as 10 nM. These compounds were also inhibitors of the HCV replicon in cultured HUH7 cells.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2QE2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Hepatitis_c_virus_subtype_1b Hepatitis c virus subtype 1b]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QE2 OCA].
</div>
<div class="pdbe-citations 2qe2" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Identification of anthranilic acid derivatives as a novel class of allosteric inhibitors of hepatitis C NS5B polymerase., Nittoli T, Curran K, Insaf S, DiGrandi M, Orlowski M, Chopra R, Agarwal A, Howe AY, Prashad A, Floyd MB, Johnson B, Sutherland A, Wheless K, Feld B, O'Connell J, Mansour TS, Bloom J, J Med Chem. 2007 May 3;50(9):2108-16. Epub 2007 Apr 3. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17402724 17402724]
*[[RNA polymerase 3D structures|RNA polymerase 3D structures]]
[[Category: Hepatitis c virus subtype 1b]]
== References ==
[[Category: RNA-directed RNA polymerase]]
<references/>
[[Category: Single protein]]
__TOC__
[[Category: Bard, J.]]
</StructureSection>
[[Category: Chopra, R.]]
[[Category: Hepatitis C virus subtype 1b]]
[[Category: Svenson, K.]]
[[Category: Large Structures]]
[[Category: Transferase]]
[[Category: Bard J]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 14:47:51 2008''
[[Category: Chopra R]]
[[Category: Svenson K]]
Retrieved from "https://proteopedia.org/2qe2"