2bx5: Difference between revisions

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New page: left|200px<br /> <applet load="2bx5" size="450" color="white" frame="true" align="right" spinBox="true" caption="2bx5, resolution 2.7Å" /> '''IS FR1 THE ANTIBODY'...
 
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[[Image:2bx5.gif|left|200px]]<br />
<applet load="2bx5" size="450" color="white" frame="true" align="right" spinBox="true"
caption="2bx5, resolution 2.7&Aring;" />
'''IS FR1 THE ANTIBODY'S ACHILLIES HEEL'''<br />


==Overview==
==Is FR1 the antibody's Achillies heel==
Antibodies are the archetypal molecules of the Ig-fold superfamily. Their, highly conserved beta-sheet architecture has evolved to avoid aggregation, by protecting edge strands. However, the crystal structure of a human V, kappa domain described here, reveals an exposed beta-edge strand which, mediates assembly of a helical pentadecameric oligomer. This edge strand, is highly conserved in V kappa domains but is both shortened and capped by, the use of two sequential trans-proline residues in V lambda domains. We, suggest that the exposure of this beta-edge in V kappa domains may explain, why light-chain deposition disease is mediated predominantly by kappa, antibodies.
<StructureSection load='2bx5' size='340' side='right'caption='[[2bx5]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2bx5]] is a 15 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BX5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BX5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bx5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bx5 OCA], [https://pdbe.org/2bx5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bx5 RCSB], [https://www.ebi.ac.uk/pdbsum/2bx5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bx5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9UL77_HUMAN Q9UL77_HUMAN]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bx/2bx5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2bx5 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Antibodies are the archetypal molecules of the Ig-fold superfamily. Their highly conserved beta-sheet architecture has evolved to avoid aggregation by protecting edge strands. However, the crystal structure of a human V kappa domain described here, reveals an exposed beta-edge strand which mediates assembly of a helical pentadecameric oligomer. This edge strand is highly conserved in V kappa domains but is both shortened and capped by the use of two sequential trans-proline residues in V lambda domains. We suggest that the exposure of this beta-edge in V kappa domains may explain why light-chain deposition disease is mediated predominantly by kappa antibodies.


==About this Structure==
Beta-edge interactions in a pentadecameric human antibody V kappa domain.,James LC, Jones PC, McCoy A, Tennent GA, Pepys MB, Famm K, Winter G J Mol Biol. 2007 Mar 30;367(3):603-8. Epub 2006 Nov 3. PMID:17292396<ref>PMID:17292396</ref>
2BX5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2BX5 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Beta-edge interactions in a pentadecameric human antibody V kappa domain., James LC, Jones PC, McCoy A, Tennent GA, Pepys MB, Famm K, Winter G, J Mol Biol. 2007 Mar 30;367(3):603-8. Epub 2006 Nov 3. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=17292396 17292396]
</div>
[[Category: Escherichia coli]]
<div class="pdbe-citations 2bx5" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
== References ==
[[Category: James, L.C.]]
<references/>
[[Category: aggregation]]
__TOC__
[[Category: amyloid]]
</StructureSection>
[[Category: antibody]]
[[Category: Homo sapiens]]
[[Category: fr1]]
[[Category: Large Structures]]
[[Category: lcdd]]
[[Category: James LC]]
[[Category: light-chain]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Sun Nov 18 09:48:09 2007''

Latest revision as of 07:47, 23 October 2024

Is FR1 the antibody's Achillies heel

2bx5, resolution 2.70Å

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