2qhr: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,331,613 edits
No edit summary
 
(13 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:2qhr.jpg|left|200px]]
<!--
The line below this paragraph, containing "STRUCTURE_2qhr", creates the "Structure Box" on the page.
You may change the PDB parameter (which sets the PDB file loaded into the applet)
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
or leave the SCENE parameter empty for the default display.
-->
{{STRUCTURE_2qhr|  PDB=2qhr  |  SCENE=  }}
'''Crystal structure of the 13F6-1-2 Fab fragment bound to its Ebola virus glycoprotein peptide epitope.'''


==Crystal structure of the 13F6-1-2 Fab fragment bound to its Ebola virus glycoprotein peptide epitope.==
<StructureSection load='2qhr' size='340' side='right'caption='[[2qhr]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2qhr]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Ebola_virus_-_Eckron_(Zaire,_1976) Ebola virus - Eckron (Zaire, 1976)] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QHR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QHR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qhr OCA], [https://pdbe.org/2qhr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qhr RCSB], [https://www.ebi.ac.uk/pdbsum/2qhr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qhr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LAC2_MOUSE LAC2_MOUSE]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qh/2qhr_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qhr ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
13F6-1-2 is a murine monoclonal antibody that recognizes the heavily glycosylated mucin-like domain of the Ebola virus virion-attached glycoprotein (GP) and protects animals against lethal viral challenge. Here we present the crystal structure, at 2.0 A, of 13F6-1-2 in complex with its Ebola virus GP peptide epitope. The GP peptide binds in an extended conformation, anchored primarily by interactions with the heavy chain. Two GP residues, Gln P406 and Arg P409, make extensive side-chain hydrogen bond and electrostatic interactions with the antibody and are likely critical for recognition and affinity. The 13F6-1-2 antibody utilizes a rare V lambda(x) light chain. The three light-chain complementarity-determining regions do not adopt canonical conformations and represent new classes of structures distinct from V kappa and other V lambda light chains. In addition, although V lambda(x) had been thought to confer specificity, all light-chain contacts are mediated through germ-line-encoded residues. This structure of an antibody that protects against the Ebola virus now provides a framework for humanization and development of a postexposure immunotherapeutic.


==Overview==
Complex of a protective antibody with its Ebola virus GP peptide epitope: unusual features of a V lambda x light chain.,Lee JE, Kuehne A, Abelson DM, Fusco ML, Hart MK, Saphire EO J Mol Biol. 2008 Jan 4;375(1):202-16. Epub 2007 Oct 16. PMID:18005986<ref>PMID:18005986</ref>
13F6-1-2 is a murine monoclonal antibody that recognizes the heavily glycosylated mucin-like domain of the Ebola virus virion-attached glycoprotein (GP) and protects animals against lethal viral challenge. Here we present the crystal structure, at 2.0 A, of 13F6-1-2 in complex with its Ebola virus GP peptide epitope. The GP peptide binds in an extended conformation, anchored primarily by interactions with the heavy chain. Two GP residues, Gln P406 and Arg P409, make extensive side-chain hydrogen bond and electrostatic interactions with the antibody and are likely critical for recognition and affinity. The 13F6-1-2 antibody utilizes a rare V lambda(x) light chain. The three light-chain complementarity-determining regions do not adopt canonical conformations and represent new classes of structures distinct from V kappa and other V lambda light chains. In addition, although V lambda(x) had been thought to confer specificity, all light-chain contacts are mediated through germ-line-encoded residues. This structure of an antibody that protects against the Ebola virus now provides a framework for humanization and development of a postexposure immunotherapeutic.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QHR OCA].
</div>
<div class="pdbe-citations 2qhr" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Complex of a protective antibody with its Ebola virus GP peptide epitope: unusual features of a V lambda x light chain., Lee JE, Kuehne A, Abelson DM, Fusco ML, Hart MK, Saphire EO, J Mol Biol. 2008 Jan 4;375(1):202-16. Epub 2007 Oct 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18005986 18005986]
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
[[Category: Abelson, D M.]]
== References ==
[[Category: Fusco, M L.]]
<references/>
[[Category: Hart, M K.]]
__TOC__
[[Category: Kuehne, A.]]
</StructureSection>
[[Category: Lee, J E.]]
[[Category: Large Structures]]
[[Category: Saphire, E O.]]
[[Category: Mus musculus]]
[[Category: Antibody-peptide complex]]
[[Category: Abelson DM]]
[[Category: Immune system/viral protein complex]]
[[Category: Fusco ML]]
[[Category: Immunologlobulin fold]]
[[Category: Hart MK]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 14:59:12 2008''
[[Category: Kuehne A]]
[[Category: Lee JE]]
[[Category: Saphire EO]]
Retrieved from "https://proteopedia.org/2qhr"