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[[Image:2r5m.gif|left|200px]]
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{{STRUCTURE_2r5m|  PDB=2r5m  |  SCENE=  }}
'''Crystal Structure of the two MBT repeats from Sex-Comb on Midleg (SCM) in complex with peptide R-(me)K-S'''


==Crystal Structure of the two MBT repeats from Sex-Comb on Midleg (SCM) in complex with peptide R-(me)K-S==
<StructureSection load='2r5m' size='340' side='right'caption='[[2r5m]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2r5m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R5M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R5M FirstGlance]. <br>
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MLZ:N-METHYL-LYSINE'>MLZ</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2r57|2r57]], [[2r58|2r58]], [[2r5a|2r5a]]</div></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Scm ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r5m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r5m OCA], [https://pdbe.org/2r5m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r5m RCSB], [https://www.ebi.ac.uk/pdbsum/2r5m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r5m ProSAT]</span></td></tr>
</table>
== Function ==
[[https://www.uniprot.org/uniprot/SCM_DROME SCM_DROME]] Polycomb group (PcG) protein. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via the methylation of histones, rendering chromatin heritably changed in its expressibility.<ref>PMID:15280237</ref> [UniProtKB:Q9W3C1]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r5/2r5m_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r5m ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sex comb on midleg (Scm) is a member of the Polycomb group of proteins involved in the maintenance of repression of Hox and other developmental control genes in Drosophila. The two malignant brain tumour (MBT) repeats of Scm form a domain that preferentially binds to monomethylated lysine residues either as a free amino acid or in the context of peptides, while unmodified or di- or trimethylated lysine residues are bound with significantly lower affinity. The crystal structure of a monomethyl-lysine-containing histone tail peptide bound to the MBT repeat domain shows that the methyl-lysine side chain occupies a binding pocket in the second MBT repeat formed by three conserved aromatic residues and one aspartate. Insertion of the monomethylated side chain into this pocket seems to be the main contributor to the binding affinity. Functional analyses in Drosophila show that the MBT domain of Scm and its methyl-lysine-binding activity are required for repression of Hox genes.


==Overview==
Structural and functional analyses of methyl-lysine binding by the malignant brain tumour repeat protein Sex comb on midleg.,Grimm C, de Ayala Alonso AG, Rybin V, Steuerwald U, Ly-Hartig N, Fischle W, Muller J, Muller CW EMBO Rep. 2007 Nov;8(11):1031-7. Epub 2007 Oct 12. PMID:17932512<ref>PMID:17932512</ref>
Sex comb on midleg (Scm) is a member of the Polycomb group of proteins involved in the maintenance of repression of Hox and other developmental control genes in Drosophila. The two malignant brain tumour (MBT) repeats of Scm form a domain that preferentially binds to monomethylated lysine residues either as a free amino acid or in the context of peptides, while unmodified or di- or trimethylated lysine residues are bound with significantly lower affinity. The crystal structure of a monomethyl-lysine-containing histone tail peptide bound to the MBT repeat domain shows that the methyl-lysine side chain occupies a binding pocket in the second MBT repeat formed by three conserved aromatic residues and one aspartate. Insertion of the monomethylated side chain into this pocket seems to be the main contributor to the binding affinity. Functional analyses in Drosophila show that the MBT domain of Scm and its methyl-lysine-binding activity are required for repression of Hox genes.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2R5M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R5M OCA].
</div>
<div class="pdbe-citations 2r5m" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structural and functional analyses of methyl-lysine binding by the malignant brain tumour repeat protein Sex comb on midleg., Grimm C, de Ayala Alonso AG, Rybin V, Steuerwald U, Ly-Hartig N, Fischle W, Muller J, Muller CW, EMBO Rep. 2007 Nov;8(11):1031-7. Epub 2007 Oct 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17932512 17932512]
*[[Polycomb|Polycomb]]
[[Category: Drosophila melanogaster]]
== References ==
[[Category: Single protein]]
<references/>
[[Category: Grimm, C.]]
__TOC__
[[Category: Mueller, C W.]]
</StructureSection>
[[Category: Steuerwald, U.]]
[[Category: Drome]]
[[Category: Large Structures]]
[[Category: Grimm, C]]
[[Category: Mueller, C W]]
[[Category: Steuerwald, U]]
[[Category: Chromatin regulator]]
[[Category: Chromatin regulator]]
[[Category: Developmental protein]]
[[Category: Developmental protein]]
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[[Category: Transcription]]
[[Category: Transcription]]
[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 16:17:13 2008''

Latest revision as of 04:15, 2 July 2021

Crystal Structure of the two MBT repeats from Sex-Comb on Midleg (SCM) in complex with peptide R-(me)K-S

2r5m, resolution 2.65Å

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