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[[Image:2vl5.jpg|left|200px]]
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{{STRUCTURE_2vl5|  PDB=2vl5  |  SCENE=  }}
'''STRUCTURE OF ANTI-COLLAGEN TYPE II FAB CIIC1'''


==Structure of anti-collagen type II FAb CIIC1==
<StructureSection load='2vl5' size='340' side='right'caption='[[2vl5]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2vl5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VL5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VL5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vl5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vl5 OCA], [https://pdbe.org/2vl5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vl5 RCSB], [https://www.ebi.ac.uk/pdbsum/2vl5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vl5 ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vl/2vl5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vl5 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Monoclonal anti-collagen type II antibody CIIC1 is an arthritogenic autoantibody, which induces arthritis in mice. We crystallized and solved the structure of CIIC1 Fab molecule. Analysis of structure revealed an interaction between the CDR regions of one Fab to the CH1 domain of another Fab, which resembles an antibody-antigen interaction. ELISA experiments confirmed the cross-reactivity of both the full CIIC1 antibody and a single chain Fv fragment to other anti-collagen antibodies which are of different isotypes and epitope specificity. The rheumatoid factor like reactivity of CIIC1 antibody together with its collagen type II specificity may explain the pathogenicity of this antibody.


==Overview==
The crystal structure of the pathogenic collagen type II-specific mouse monoclonal antibody CIIC1 Fab: structure to function analysis.,Uysal H, Sehnert B, Nandakumar KS, Boiers U, Burkhardt H, Holmdahl R, Thunnissen MM Mol Immunol. 2008 Apr;45(8):2196-204. Epub 2008 Feb 1. PMID:18241923<ref>PMID:18241923</ref>
Monoclonal anti-collagen type II antibody CIIC1 is an arthritogenic autoantibody, which induces arthritis in mice. We crystallized and solved the structure of CIIC1 Fab molecule. Analysis of structure revealed an interaction between the CDR regions of one Fab to the CH1 domain of another Fab, which resembles an antibody-antigen interaction. ELISA experiments confirmed the cross-reactivity of both the full CIIC1 antibody and a single chain Fv fragment to other anti-collagen antibodies which are of different isotypes and epitope specificity. The rheumatoid factor like reactivity of CIIC1 antibody together with its collagen type II specificity may explain the pathogenicity of this antibody.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2VL5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VL5 OCA].
</div>
<div class="pdbe-citations 2vl5" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
The crystal structure of the pathogenic collagen type II-specific mouse monoclonal antibody CIIC1 Fab: Structure to function analysis., Uysal H, Sehnert B, Nandakumar KS, Boiers U, Burkhardt H, Holmdahl R, Thunnissen MM, Mol Immunol. 2008 Apr;45(8):2196-204. Epub 2008 Feb 1. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18241923 18241923]
*[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Boiers U]]
[[Category: Boiers, U.]]
[[Category: Burkhardt H]]
[[Category: Burkhardt, H.]]
[[Category: Holmdahl R]]
[[Category: Holmdahl, R.]]
[[Category: Nandakumar KS]]
[[Category: Nandakumar, K S.]]
[[Category: Sehnert B]]
[[Category: Sehnert, B.]]
[[Category: Thunnissen MM]]
[[Category: Thunnissen, M M.]]
[[Category: Uysal H]]
[[Category: Uysal, H.]]
[[Category: Arthritis]]
[[Category: Autoantibody]]
[[Category: Collagen type ii]]
[[Category: Immune system]]
[[Category: Rheumatoid factor]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 18:59:02 2008''
Retrieved from "https://proteopedia.org/2vl5"