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New page: left|200px<br /><applet load="1add" size="450" color="white" frame="true" align="right" spinBox="true" caption="1add, resolution 2.4Å" /> '''A PRE-TRANSITION STAT...
 
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[[Image:1add.gif|left|200px]]<br /><applet load="1add" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1add, resolution 2.4&Aring;" />
'''A PRE-TRANSITION STATE MIMIC OF AN ENZYME: X-RAY STRUCTURE OF ADENOSINE DEAMINASE WITH BOUND 1-DEAZA-ADENOSINE AND ZINC-ACTIVATED WATER'''<br />


==Overview==
==A PRE-TRANSITION STATE MIMIC OF AN ENZYME: X-RAY STRUCTURE OF ADENOSINE DEAMINASE WITH BOUND 1-DEAZA-ADENOSINE AND ZINC-ACTIVATED WATER==
The refined 2.4-A structure of adenosine deaminase, recently discovered to, be a zinc metalloenzyme [Wilson, D. K., Rudolph, F. B., &amp; Quiocho, F. A., (1991) Science 252, 1278-1284], complexed with the ground-state analog, 1-deazaadenosine shows the mode of binding of the analog and, unexpectedly, a zinc-activated water (hydroxide). This structure of a, pre-transition-state mimic, combined with that previously determined for, the complex with 6(R)-hydroxy-1,6-dihydropurine ribonucleoside, a nearly, ideal transition-state analog, sheds new understanding of the precise, stereospecificity and hydrolytic catalysis of an important and, well-characterized member of a large group of zinc metalloenzymes. As both, of these excellent mimics were generated in the active site, they, demonstrate a powerful means of dissecting the course of an enzymatic, reaction by direct crystallographic analysis.
<StructureSection load='1add' size='340' side='right'caption='[[1add]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1add]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ADD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ADD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1DA:1-DEAZA-ADENOSINE'>1DA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1add FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1add OCA], [https://pdbe.org/1add PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1add RCSB], [https://www.ebi.ac.uk/pdbsum/1add PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1add ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ADA_MOUSE ADA_MOUSE] Catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. Plays an important role in purine metabolism and in adenosine homeostasis. Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (By similarity).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ad/1add_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1add ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1ADD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] with ZN and 1DA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Adenosine_deaminase Adenosine deaminase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.4.4 3.5.4.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ADD OCA].
*[[Adenosine deaminase 3D structures|Adenosine deaminase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
A pre-transition-state mimic of an enzyme: X-ray structure of adenosine deaminase with bound 1-deazaadenosine and zinc-activated water., Wilson DK, Quiocho FA, Biochemistry. 1993 Feb 23;32(7):1689-94. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8439534 8439534]
[[Category: Large Structures]]
[[Category: Adenosine deaminase]]
[[Category: Mus musculus]]
[[Category: Mus musculus]]
[[Category: Single protein]]
[[Category: Quiocho FA]]
[[Category: Quiocho, F.A.]]
[[Category: Wilson DK]]
[[Category: Wilson, D.K.]]
[[Category: 1DA]]
[[Category: ZN]]
[[Category: hydrolase(acting in cyclicamidines)]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:46:15 2007''

Latest revision as of 06:29, 7 February 2024

A PRE-TRANSITION STATE MIMIC OF AN ENZYME: X-RAY STRUCTURE OF ADENOSINE DEAMINASE WITH BOUND 1-DEAZA-ADENOSINE AND ZINC-ACTIVATED WATER

1add, resolution 2.40Å

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