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New page: left|200px<br /><applet load="1af5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1af5, resolution 3.00Å" /> '''GROUP I MOBILE INTRO...
 
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[[Image:1af5.gif|left|200px]]<br /><applet load="1af5" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1af5, resolution 3.00&Aring;" />
'''GROUP I MOBILE INTRON ENDONUCLEASE'''<br />


==Overview==
==GROUP I MOBILE INTRON ENDONUCLEASE==
The structure of I-Crel provides the first view of a protein encoded by a, gene within an intron. This endonuclease recognizes a long DNA site, approximately 20 base pairs in length and facilitates the lateral transfer, of that intron. The protein exhibits a DNA-binding surface consisting of, four antiparallel beta-strands that form a 20 A wide groove which is over, 70 A long. The architecture of this fold is different from that of the, TATA binding protein, TBP, which also contains an antiparallel, beta-saddle. The conserved LAGLIDADG motif, which is found in many mobile, intron endonucleases, maturases and inteins, forms a novel helical, interface and contributes essential residues to the active site.
<StructureSection load='1af5' size='340' side='right'caption='[[1af5]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1af5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AF5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AF5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1af5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1af5 OCA], [https://pdbe.org/1af5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1af5 RCSB], [https://www.ebi.ac.uk/pdbsum/1af5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1af5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/af/1af5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1af5 ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1AF5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AF5 OCA].
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The structure of I-Crel, a group I intron-encoded homing endonuclease., Heath PJ, Stephens KM, Monnat RJ Jr, Stoddard BL, Nat Struct Biol. 1997 Jun;4(6):468-76. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9187655 9187655]
[[Category: Chlamydomonas reinhardtii]]
[[Category: Chlamydomonas reinhardtii]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Heath, P.J.]]
[[Category: Heath PJ]]
[[Category: Junior, R.J.Monnat.]]
[[Category: Monnat Junior RJ]]
[[Category: Stephens, K.M.]]
[[Category: Stephens KM]]
[[Category: Stoddard, B.L.]]
[[Category: Stoddard BL]]
[[Category: chloroplast dna]]
[[Category: endonuclease]]
[[Category: group i mobile intron]]
[[Category: intron homing]]
[[Category: laglidadg motif]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 10:48:23 2007''

Latest revision as of 06:30, 7 February 2024

GROUP I MOBILE INTRON ENDONUCLEASE

1af5, resolution 3.00Å

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