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| <!--
| | ==STRUCTURE OF THE SIDE-BY-SIDE BINDING OF DISTAMYCIN TO DNA== |
| The line below this paragraph, containing "STRUCTURE_378d", creates the "Structure Box" on the page.
| | <StructureSection load='378d' size='340' side='right'caption='[[378d]], [[Resolution|resolution]] 2.40Å' scene=''> |
| You may change the PDB parameter (which sets the PDB file loaded into the applet)
| | == Structural highlights == |
| or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| | <table><tr><td colspan='2'>[[378d]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=378D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=378D FirstGlance]. <br> |
| or leave the SCENE parameter empty for the default display.
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| -->
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DMY:DISTAMYCIN+A'>DMY</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| {{STRUCTURE_378d| PDB=378d | SCENE= }}
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=378d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=378d OCA], [https://pdbe.org/378d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=378d RCSB], [https://www.ebi.ac.uk/pdbsum/378d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=378d ProSAT]</span></td></tr> |
| | | </table> |
| '''STRUCTURE OF THE SIDE-BY-SIDE BINDING OF DISTAMYCIN TO DNA'''
| | __TOC__ |
| | | </StructureSection> |
| | | [[Category: Large Structures]] |
| ==Overview== | | [[Category: Mitra SN]] |
| The 2.40 A resolution crystal structure of a side-by-side binding of distamycin A molecules to a DNA octamer d(GTATATAC)2 with an extended alternating TA sequence has been determined. The unit-cell parameters are a = 29.55, b = 42.18, c = 43.38 A, beta = 96.56 degrees, space group P21, with two molecules in the asymmetric unit, in contrast to all previous side-by-side distamycin-DNA complexes which have only a single DNA strand and one drug molecule in the asymmetric unit. The structure was solved by the molecular-replacement method and refined to an R index of 21.0% using 3467 reflections [>/= 2sigma(F)]. The minor grooves of the DNA molecules bind two side-by-side antiparallel staggered distamycins spanning about five base pairs and virtually covering the entire length of the DNA. The octamer duplexes exhibit low-high alternations in the helical twist, sugar puckering and the C-O3' and O3'-P torsion angles, similar to the earlier side-by-side complexes containing inosine bases. The molecules are stacked one over the other along the ac diagonal in an infinite pseudo-continuous helical column with no lateral interactions.
| | [[Category: Sundaralingam M]] |
| | | [[Category: Wahl MC]] |
| ==About this Structure== | |
| Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=378D OCA]. | |
| | |
| ==Reference== | |
| Structure of the side-by-side binding of distamycin to d(GTATATAC)2., Mitra SN, Wahl MC, Sundaralingam M, Acta Crystallogr D Biol Crystallogr. 1999 Mar;55(Pt 3):602-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10089456 10089456]
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| [[Category: Mitra, S N.]] | |
| [[Category: Sundaralingam, M.]]
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| [[Category: Wahl, M C.]] | |
| [[Category: Double drug in minor groove]] | |
| [[Category: Double helix]] | |
| [[Category: Right handed dna]] | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 20:17:56 2008''
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