3b62: Difference between revisions

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[[Image:3b62.jpg|left|200px]]


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==EmrE multidrug transporter in complex with P4P, P21 crystal form==
The line below this paragraph, containing "STRUCTURE_3b62", creates the "Structure Box" on the page.
<StructureSection load='3b62' size='340' side='right'caption='[[3b62]], [[Resolution|resolution]] 4.40&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[3b62]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B62 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3B62 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.4&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P4P:TETRAPHENYLPHOSPHONIUM'>P4P</scene></td></tr>
{{STRUCTURE_3b62|  PDB=3b62  |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3b62 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3b62 OCA], [https://pdbe.org/3b62 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3b62 RCSB], [https://www.ebi.ac.uk/pdbsum/3b62 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3b62 ProSAT]</span></td></tr>
 
</table>
'''EmrE multidrug transporter in complex with P4P, P21 crystal form'''
== Function ==
 
[https://www.uniprot.org/uniprot/EMRE_ECOLI EMRE_ECOLI] Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistance of E.coli cells to methyl viologen, ethidium bromide and acriflavine. Is also able to transport tetraphenylphosphonium (TPP(+)) and benzalkonium.<ref>PMID:7896833</ref> <ref>PMID:10681497</ref> <ref>PMID:15371426</ref>
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
EmrE, a multidrug transporter from Escherichia coli, functions as a homodimer of a small four-transmembrane protein. The membrane insertion topology of the two monomers is controversial. Although the EmrE protein was reported to have a unique orientation in the membrane, models based on electron microscopy and now defunct x-ray structures, as well as recent biochemical studies, posit an antiparallel dimer. We have now reanalyzed our x-ray data on EmrE. The corrected structures in complex with a transport substrate are highly similar to the electron microscopy structure. The first three transmembrane helices from each monomer surround the substrate binding chamber, whereas the fourth helices participate only in dimer formation. Selenomethionine markers clearly indicate an antiparallel orientation for the monomers, supporting a "dual topology" model.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b6/3b62_consurf.spt"</scriptWhenChecked>
3B62 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3B62 OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
X-ray structure of EmrE supports dual topology model., Chen YJ, Pornillos O, Lieu S, Ma C, Chen AP, Chang G, Proc Natl Acad Sci U S A. 2007 Nov 27;104(48):18999-9004. Epub 2007 Nov, 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18024586 18024586]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3b62 ConSurf].
[[Category: Escherichia coli]]
<div style="clear:both"></div>
[[Category: Single protein]]
== References ==
[[Category: Chang, G.]]
<references/>
[[Category: Chen, Y J.]]
__TOC__
[[Category: Antiport]]
</StructureSection>
[[Category: Helical membrane protein]]
[[Category: Escherichia coli K-12]]
[[Category: Inner membrane]]
[[Category: Large Structures]]
[[Category: Multidrug resistance transporter]]
[[Category: Chang G]]
[[Category: Smr]]
[[Category: Chen YJ]]
[[Category: Transmembrane]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 20:25:53 2008''

Latest revision as of 09:27, 21 February 2024

EmrE multidrug transporter in complex with P4P, P21 crystal form

3b62, resolution 4.40Å

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