1aua: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
New page: left|200px<br /><applet load="1aua" size="450" color="white" frame="true" align="right" spinBox="true" caption="1aua, resolution 2.5Å" /> '''PHOSPHATIDYLINOSITOL ...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
 
(15 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1aua.gif|left|200px]]<br /><applet load="1aua" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1aua, resolution 2.5&Aring;" />
'''PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SEC14P FROM SACCHAROMYCES CEREVISIAE'''<br />


==Overview==
==PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SEC14P FROM SACCHAROMYCES CEREVISIAE==
The yeast phosphatidylinositol-transfer protein (Sec14) catalyses exchange, of phosphatidylinositol and phosphatidylcholine between membrane bilayers, in vitro. In vivo, Sec14 activity is essential for vesicle budding from, the Golgi complex. Here we report a three-dimensional structure for Sec14, at 2.5 A resolution. Sec14 consists of twelve alpha-helices, six, beta-strands, eight 3(10)-helices and has two distinct domains. The, carboxy-terminal domain forms a hydrophobic pocket which, in the crystal, structure, is occupied by two molecules of n-octyl-beta-D-glucopyranoside, and represents the phospholipid-binding domain. This pocket is reinforced, by a string motif whose disruption in a sec14 temperature-sensitive mutant, results in destabilization of the phospholipid-binding domain. Finally, we, have identified an unusual surface helix that may play a critical role in, driving Sec14-mediated phospholipid exchange. From this structure, we, derive the first molecular clues into how a phosphatidylinositol-transfer, protein functions.
<StructureSection load='1aua' size='340' side='right'caption='[[1aua]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1aua]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AUA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AUA FirstGlance]. <br>
1AUA is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with BOG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AUA OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aua FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aua OCA], [https://pdbe.org/1aua PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aua RCSB], [https://www.ebi.ac.uk/pdbsum/1aua PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aua ProSAT]</span></td></tr>
Crystal structure of the Saccharomyces cerevisiae phosphatidylinositol-transfer protein., Sha B, Phillips SE, Bankaitis VA, Luo M, Nature. 1998 Jan 29;391(6666):506-10. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9461221 9461221]
</table>
== Function ==
[https://www.uniprot.org/uniprot/SEC14_YEAST SEC14_YEAST] Required for transport of secretory proteins from the Golgi complex. Catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes in vitro. Essential for viability and secretion.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/au/1aua_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aua ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Bankaitis VA]]
[[Category: Bankaitis, V.A.]]
[[Category: Luo M]]
[[Category: Luo, M.]]
[[Category: Phillips SE]]
[[Category: Phillips, S.E.]]
[[Category: Sha B]]
[[Category: Sha, B.]]
[[Category: BOG]]
[[Category: golgi-derived secretory vesicle biogenesis]]
[[Category: peripheral golgi membrane protein]]
[[Category: phospholipid exchange]]
[[Category: phospholipid-binding protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:08:13 2007''

Latest revision as of 06:33, 7 February 2024

PHOSPHATIDYLINOSITOL TRANSFER PROTEIN SEC14P FROM SACCHAROMYCES CEREVISIAE

1aua, resolution 2.50Å

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "https://proteopedia.org/index.php?title=1aua&oldid=4040346"