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[[Image:3sgb.jpg|left|200px]]
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{{STRUCTURE_3sgb|  PDB=3sgb  |  SCENE=  }}
'''STRUCTURE OF THE COMPLEX OF STREPTOMYCES GRISEUS PROTEASE B AND THE THIRD DOMAIN OF THE TURKEY OVOMUCOID INHIBITOR AT 1.8 ANGSTROMS RESOLUTION'''


==STRUCTURE OF THE COMPLEX OF STREPTOMYCES GRISEUS PROTEASE B AND THE THIRD DOMAIN OF THE TURKEY OVOMUCOID INHIBITOR AT 1.8 ANGSTROMS RESOLUTION==
<StructureSection load='3sgb' size='340' side='right'caption='[[3sgb]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[3sgb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_griseus"_krainsky_1914 "actinomyces griseus" krainsky 1914]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=2sgb 2sgb] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1sgb 1sgb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SGB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3SGB FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3sgb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3sgb OCA], [http://pdbe.org/3sgb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3sgb RCSB], [http://www.ebi.ac.uk/pdbsum/3sgb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3sgb ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/PRTB_STRGR PRTB_STRGR]] Has a primary specificity for large aliphatic or aromatic amino acids.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sg/3sgb_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3sgb ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of the complex between the serine protease Streptomyces griseus protease B (SGPB) and the third domain of the Kazal-type ovomucoid inhibitor from turkey has been solved at 1.8-A resolution and refined to a conventional R factor of 0.125. As others have reported previously for analogous complexes of proteases and protein inhibitors, the inhibitor binds in a fashion similar to that of a substrate; it is not cleaved, but there is a close approach (2.7 A) of the active site nucleophile Ser-195 O gamma to the carbonyl carbon of the reactive peptide bond of the inhibitor. Contrary to the structural reports regarding the other enzyme-inhibitor complexes, we conclude that there is no evidence for a significant distortion of this peptide bond from planarity. The mechanism of inhibition can be understood in terms of the equilibrium thermodynamic parameters Ka, the enzyme-inhibitor association constant, and Khyd, the equilibrium constant for inhibitor hydrolysis. These thermodynamic parameters can be rationalized in terms of the observed structure.


==Overview==
Structure of the complex of Streptomyces griseus protease B and the third domain of the turkey ovomucoid inhibitor at 1.8-A resolution.,Read RJ, Fujinaga M, Sielecki AR, James MN Biochemistry. 1983 Sep 13;22(19):4420-33. PMID:6414511<ref>PMID:6414511</ref>
The structure of the complex between the serine protease Streptomyces griseus protease B (SGPB) and the third domain of the Kazal-type ovomucoid inhibitor from turkey has been solved at 1.8-A resolution and refined to a conventional R factor of 0.125. As others have reported previously for analogous complexes of proteases and protein inhibitors, the inhibitor binds in a fashion similar to that of a substrate; it is not cleaved, but there is a close approach (2.7 A) of the active site nucleophile Ser-195 O gamma to the carbonyl carbon of the reactive peptide bond of the inhibitor. Contrary to the structural reports regarding the other enzyme-inhibitor complexes, we conclude that there is no evidence for a significant distortion of this peptide bond from planarity. The mechanism of inhibition can be understood in terms of the equilibrium thermodynamic parameters Ka, the enzyme-inhibitor association constant, and Khyd, the equilibrium constant for inhibitor hydrolysis. These thermodynamic parameters can be rationalized in terms of the observed structure.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
3SGB is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Streptomyces_griseus Streptomyces griseus]. This structure supersedes the now removed PDB entries  and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1sgb 1sgb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3SGB OCA].
</div>
<div class="pdbe-citations 3sgb" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Structure of the complex of Streptomyces griseus protease B and the third domain of the turkey ovomucoid inhibitor at 1.8-A resolution., Read RJ, Fujinaga M, Sielecki AR, James MN, Biochemistry. 1983 Sep 13;22(19):4420-33. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/6414511 6414511]
*[[Proteinase|Proteinase]]
[[Category: Protein complex]]
*[[Proteinase 3D structures|Proteinase 3D structures]]
[[Category: Streptomyces griseus]]
== References ==
[[Category: Fujinaga, M.]]
<references/>
[[Category: James, M N.G.]]
__TOC__
[[Category: Read, R J.]]
</StructureSection>
[[Category: Sielecki, A R.]]
[[Category: Actinomyces griseus krainsky 1914]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 22:13:06 2008''
[[Category: Large Structures]]
[[Category: Fujinaga, M]]
[[Category: James, M N.G]]
[[Category: Read, R J]]
[[Category: Sielecki, A R]]

Latest revision as of 13:18, 1 January 2020

STRUCTURE OF THE COMPLEX OF STREPTOMYCES GRISEUS PROTEASE B AND THE THIRD DOMAIN OF THE TURKEY OVOMUCOID INHIBITOR AT 1.8 ANGSTROMS RESOLUTION

3sgb, resolution 1.80Å

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