1ayi: Difference between revisions

From Proteopedia
Jump to navigationJump to search
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
New page: left|200px<br /><applet load="1ayi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ayi, resolution 2.0Å" /> '''COLICIN E7 IMMUNITY P...
 
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,330,058 edits
No edit summary
 
(17 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1ayi.gif|left|200px]]<br /><applet load="1ayi" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1ayi, resolution 2.0&Aring;" />
'''COLICIN E7 IMMUNITY PROTEIN IM7'''<br />


==Overview==
==COLICIN E7 IMMUNITY PROTEIN IM7==
We report the first detailed comparison of two immunity proteins which, in, conjunction with recent protein engineering data, begins to explain how, these structurally similar proteins are able to bind and inhibit the, endonuclease domain of colicin E9 (E9 DNase) with affinities that differ, by 12 orders of magnitude. In the present work, we have determined the, X-ray structure of the Escherichia coli colicin E7 immunity protein Im7 to, 2.0 A resolution by molecular replacement, using as a trial model the, recently determined NMR solution structure of Im9. Whereas the two, proteins adopt similar four-helix structures, subtle structural, differences, in particular involving a conserved tyrosine residue critical, for E9 DNase binding, and the identity of key residues in the specificity, helix, lie at the heart of their markedly different ability to bind the E9, DNase. Two other crystal structures were reported recently for Im7; in, one, Im7 was a monomer and was very similar to the structure reported, here, whereas in the other it was a dimer to which functional significance, was assigned. Since this previous work suggested that Im7 could exist, either as a monomer or a dimer, we used analytical ultracentrifugation to, investigate this question further. Under a variety of solution conditions, we found that Im7 only ever exists in solution as a monomer, even up to, protein concentrations of 15 mg/ml, casting doubt on the functional, significance of the crystallographically observed dimer. This work, provides a structural framework with which we can understand, immunity-protein specificity, and in addition we believe it to be the, first successfully refined crystal structure solved by molecular, replacement using an NMR trial model with less than 100% sequence, identity.
<StructureSection load='1ayi' size='340' side='right'caption='[[1ayi]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1ayi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AYI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ayi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ayi OCA], [https://pdbe.org/1ayi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ayi RCSB], [https://www.ebi.ac.uk/pdbsum/1ayi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ayi ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IMM7_ECOLX IMM7_ECOLX] This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ay/1ayi_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ayi ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1AYI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1AYI OCA].
*[[Colicin immunity protein 3D structures|Colicin immunity protein 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
A structural comparison of the colicin immunity proteins Im7 and Im9 gives new insights into the molecular determinants of immunity-protein specificity., Dennis CA, Videler H, Pauptit RA, Wallis R, James R, Moore GR, Kleanthous C, Biochem J. 1998 Jul 1;333 ( Pt 1):183-91. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9639578 9639578]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Dennis, C.A.]]
[[Category: Dennis CA]]
[[Category: James, R.]]
[[Category: James R]]
[[Category: Kleanthous, C.]]
[[Category: Kleanthous C]]
[[Category: Moore, G.R.]]
[[Category: Moore GR]]
[[Category: Pauptit, R.A.]]
[[Category: Pauptit RA]]
[[Category: Wallis, R.]]
[[Category: Wallis R]]
[[Category: bacteriocin]]
[[Category: dnase inhibitor]]
[[Category: e colicins]]
[[Category: protein-protein interactions]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:12:32 2007''
Retrieved from "https://proteopedia.org/1ayi"