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[[Image:8drh.jpg|left|200px]]


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==HIGH RESOLUTION NMR STRUCTURE OF THE D(GCGTCAGG)R(CCUGACGC) HYBRID, MINIMIZED AVERAGE STRUCTURE==
The line below this paragraph, containing "STRUCTURE_8drh", creates the "Structure Box" on the page.
<StructureSection load='8drh' size='340' side='right'caption='[[8drh]]' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[8drh]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DRH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8DRH FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8drh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8drh OCA], [https://pdbe.org/8drh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8drh RCSB], [https://www.ebi.ac.uk/pdbsum/8drh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8drh ProSAT]</span></td></tr>
{{STRUCTURE_8drh|  PDB=8drh  |  SCENE= }}
</table>
 
<div style="background-color:#fffaf0;">
'''HIGH RESOLUTION NMR STRUCTURE OF THE D(GCGTCAGG)R(CCUGACGC) HYBRID, MINIMIZED AVERAGE STRUCTURE'''
== Publication Abstract from PubMed ==
 
 
==Overview==
In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular Rp-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-Rp)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H.
In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular Rp-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-Rp)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H.


==About this Structure==
Structure of a stereoregular phosphorothioate DNA/RNA duplex.,Bachelin M, Hessler G, Kurz G, Hacia JG, Dervan PB, Kessler H Nat Struct Biol. 1998 Apr;5(4):271-6. PMID:9546216<ref>PMID:9546216</ref>
Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8DRH OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure of a stereoregular phosphorothioate DNA/RNA duplex., Bachelin M, Hessler G, Kurz G, Hacia JG, Dervan PB, Kessler H, Nat Struct Biol. 1998 Apr;5(4):271-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9546216 9546216]
</div>
[[Category: Bachelin, M.]]
<div class="pdbe-citations 8drh" style="background-color:#fffaf0;"></div>
[[Category: Dervan, P B.]]
== References ==
[[Category: Hacia, J G.]]
<references/>
[[Category: Hessler, G.]]
__TOC__
[[Category: Kessler, H.]]
</StructureSection>
[[Category: Kurz, G.]]
[[Category: Large Structures]]
[[Category: Deoxyribonucleic acid/ribonucleic acid]]
[[Category: Bachelin M]]
[[Category: Dna/rna hybrid]]
[[Category: Dervan PB]]
[[Category: Oligonucleotide]]
[[Category: Hacia JG]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May  4 22:49:12 2008''
[[Category: Hessler G]]
[[Category: Kessler H]]
[[Category: Kurz G]]
Retrieved from "https://proteopedia.org/8drh"