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{{STRUCTURE_2vqi|  PDB=2vqi  |  SCENE=  }}
'''STRUCTURE OF THE P PILUS USHER (PAPC) TRANSLOCATION PORE'''


==Structure of the P pilus usher (PapC) translocation pore==
<StructureSection load='2vqi' size='340' side='right'caption='[[2vqi]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2vqi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VQI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VQI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C8E:(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE'>C8E</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vqi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vqi OCA], [https://pdbe.org/2vqi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vqi RCSB], [https://www.ebi.ac.uk/pdbsum/2vqi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vqi ProSAT]</span></td></tr>
</table>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vq/2vqi_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vqi ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Gram-negative pathogens commonly exhibit adhesive pili on their surfaces that mediate specific attachment to the host. A major class of pili is assembled via the chaperone/usher pathway. Here, the structural basis for pilus fiber assembly and secretion performed by the outer membrane assembly platform--the usher--is revealed by the crystal structure of the translocation domain of the P pilus usher PapC and single particle cryo-electron microscopy imaging of the FimD usher bound to a translocating type 1 pilus assembly intermediate. These structures provide molecular snapshots of a twinned-pore translocation machinery in action. Unexpectedly, only one pore is used for secretion, while both usher protomers are used for chaperone-subunit complex recruitment. The translocating pore itself comprises 24 beta strands and is occluded by a folded plug domain, likely gated by a conformationally constrained beta-hairpin. These structures capture the secretion of a virulence factor across the outer membrane of gram-negative bacteria.


==Overview==
Fiber formation across the bacterial outer membrane by the chaperone/usher pathway.,Remaut H, Tang C, Henderson NS, Pinkner JS, Wang T, Hultgren SJ, Thanassi DG, Waksman G, Li H Cell. 2008 May 16;133(4):640-52. PMID:18485872<ref>PMID:18485872</ref>
Gram-negative pathogens commonly exhibit adhesive pili on their surfaces that mediate specific attachment to the host. A major class of pili is assembled via the chaperone/usher pathway. Here, the structural basis for pilus fiber assembly and secretion performed by the outer membrane assembly platform--the usher--is revealed by the crystal structure of the translocation domain of the P pilus usher PapC and single particle cryo-electron microscopy imaging of the FimD usher bound to a translocating type 1 pilus assembly intermediate. These structures provide molecular snapshots of a twinned-pore translocation machinery in action. Unexpectedly, only one pore is used for secretion, while both usher protomers are used for chaperone-subunit complex recruitment. The translocating pore itself comprises 24 beta strands and is occluded by a folded plug domain, likely gated by a conformationally constrained beta-hairpin. These structures capture the secretion of a virulence factor across the outer membrane of gram-negative bacteria.


==About this Structure==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
2VQI is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VQI OCA].
</div>
<div class="pdbe-citations 2vqi" style="background-color:#fffaf0;"></div>


==Reference==
==See Also==
Fiber formation across the bacterial outer membrane by the chaperone/usher pathway., Remaut H, Tang C, Henderson NS, Pinkner JS, Wang T, Hultgren SJ, Thanassi DG, Waksman G, Li H, Cell. 2008 May 16;133(4):640-52. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18485872 18485872]
*[[Adhesin 3D structures|Adhesin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Henderson, N S.]]
[[Category: Henderson NS]]
[[Category: Hultgren, S J.]]
[[Category: Hultgren SJ]]
[[Category: Li, H.]]
[[Category: Li H]]
[[Category: Pinkner, J S.]]
[[Category: Pinkner JS]]
[[Category: Remaut, H.]]
[[Category: Remaut H]]
[[Category: Tang, C.]]
[[Category: Tang C]]
[[Category: Thanassi, D G.]]
[[Category: Thanassi DG]]
[[Category: Waksman, G.]]
[[Category: Waksman G]]
[[Category: Wang, T.]]
[[Category: Wang T]]
[[Category: Fimbrium]]
[[Category: Membrane]]
[[Category: Om translocation pore]]
[[Category: Outer membrane]]
[[Category: P pilus]]
[[Category: Pilus assembly platform]]
[[Category: Transmembrane]]
[[Category: Transport]]
[[Category: Usher]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed May 28 09:16:41 2008''
Retrieved from "https://proteopedia.org/2vqi"