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New page: left|200px<br /><applet load="1bex" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bex, resolution 2.3Å" /> '''STRUCTURE OF RUTHENIU...
 
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[[Image:1bex.gif|left|200px]]<br /><applet load="1bex" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1bex, resolution 2.3&Aring;" />
'''STRUCTURE OF RUTHENIUM-MODIFIED PSEUDOMONAS AERUGINOSA AZURIN'''<br />


==Overview==
==STRUCTURE OF RUTHENIUM-MODIFIED PSEUDOMONAS AERUGINOSA AZURIN==
The crystal structure of Ru(2, 2'-bipyridine)2(imidazole)(His83)azurin, (RuAz) has been determined to 2.3 A -resolution by X-ray crystallography., The spectroscopic and thermodynamic properties of both the native protein, and [Ru(2, 2'-bipyridine)2(imidazole)2]2+ are maintained in the modified, protein. Dark-green RuAz crystals grown from PEG 4000, LiNO3, CuCl2 and, Tris buffer are monoclinic, belong to the space group C2 and have cell, parameters a = 100.6, b = 35.4, c = 74.7 A and beta = 106. 5 degrees. In, addition, [Ru(2,2'-bipyridine)2(imidazole)2]SO4 x 10H2O was synthesized, crystallized and structurally characterized by X-ray crystallography., Red-brown crystals of this complex are monoclinic, space group P21/n, unit-cell parameters a = 13.230 (2), b = 18.197 (4), c = 16.126 (4) A, beta = 108.65 (2) degrees. Stereochemical parameters for the refinement of, Ru(2, 2'-bipyridine)2(imidazole)(His83) were taken from the atomic, coordinates of [Ru(2,2'-bipyridine)2(imidazole)2]2+. The structure of RuAz, confirms that His83 is the only site of chemical modification and that the, native azurin structure is not perturbed significantly by the ruthenium, label.
<StructureSection load='1bex' size='340' side='right'caption='[[1bex]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1bex]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BEX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BEX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=RBU:RUTHEMIUM+BIS(2,2-BIPYRIDINE)-2-IMIDAZOLE'>RBU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bex FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bex OCA], [https://pdbe.org/1bex PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bex RCSB], [https://www.ebi.ac.uk/pdbsum/1bex PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bex ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AZUR_PSEAE AZUR_PSEAE] Transfers electrons from cytochrome c551 to cytochrome oxidase.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/be/1bex_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bex ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of Ru(2, 2'-bipyridine)2(imidazole)(His83)azurin (RuAz) has been determined to 2.3 A -resolution by X-ray crystallography. The spectroscopic and thermodynamic properties of both the native protein and [Ru(2, 2'-bipyridine)2(imidazole)2]2+ are maintained in the modified protein. Dark-green RuAz crystals grown from PEG 4000, LiNO3, CuCl2 and Tris buffer are monoclinic, belong to the space group C2 and have cell parameters a = 100.6, b = 35.4, c = 74.7 A and beta = 106. 5 degrees. In addition, [Ru(2,2'-bipyridine)2(imidazole)2]SO4 x 10H2O was synthesized, crystallized and structurally characterized by X-ray crystallography. Red-brown crystals of this complex are monoclinic, space group P21/n, unit-cell parameters a = 13.230 (2), b = 18.197 (4), c = 16.126 (4) A, beta = 108.65 (2) degrees. Stereochemical parameters for the refinement of Ru(2, 2'-bipyridine)2(imidazole)(His83) were taken from the atomic coordinates of [Ru(2,2'-bipyridine)2(imidazole)2]2+. The structure of RuAz confirms that His83 is the only site of chemical modification and that the native azurin structure is not perturbed significantly by the ruthenium label.


==About this Structure==
Structures of ruthenium-modified Pseudomonas aeruginosa azurin and [Ru(2,2'-bipyridine)2(imidazole)2]SO4 x 10H2O.,Faham S, Day MW, Connick WB, Crane BR, Di Bilio AJ, Schaefer WP, Rees DC, Gray HB Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):379-85. PMID:10089343<ref>PMID:10089343</ref>
1BEX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa] with CU and RBU as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BEX OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structures of ruthenium-modified Pseudomonas aeruginosa azurin and [Ru(2,2'-bipyridine)2(imidazole)2]SO4 x 10H2O., Faham S, Day MW, Connick WB, Crane BR, Di Bilio AJ, Schaefer WP, Rees DC, Gray HB, Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):379-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10089343 10089343]
</div>
<div class="pdbe-citations 1bex" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Azurin 3D structures|Azurin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Pseudomonas aeruginosa]]
[[Category: Single protein]]
[[Category: Day MW]]
[[Category: Day, M.W.]]
[[Category: Faham S]]
[[Category: Faham, S.]]
[[Category: Rees DC]]
[[Category: Rees, D.C.]]
[[Category: CU]]
[[Category: RBU]]
[[Category: azurin]]
[[Category: electron transport]]
 
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Latest revision as of 05:28, 13 August 2026

STRUCTURE OF RUTHENIUM-MODIFIED PSEUDOMONAS AERUGINOSA AZURIN

1bex, resolution 2.30Å

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