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New page: left|200px<br /><applet load="1bjj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bjj, resolution 2.8Å" /> '''AGKISTRODOTOXIN, A PH...
 
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[[Image:1bjj.gif|left|200px]]<br /><applet load="1bjj" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1bjj, resolution 2.8&Aring;" />
'''AGKISTRODOTOXIN, A PHOSPHOLIPASE A2-TYPE PRESYNAPTIC NEUROTOXIN FROM AGKISTRODON HALYS PALLAS'''<br />


==Overview==
==AGKISTRODOTOXIN, A PHOSPHOLIPASE A2-TYPE PRESYNAPTIC NEUROTOXIN FROM AGKISTRODON HALYS PALLAS==
The structure of agkistrodotoxin crystallized under basic conditions has, been determined at 2.8 A resolution by the molecular-replacement technique, and refined to a crystallographic R factor of 0.194 and a free R factor of, 0.260 with good stereochemistry. The molecular packing in the crystal, differs from other PLA(2)s. The six molecules in the asymmetric unit form, three dimers linked by Ca(2+) ions in a near-perfect six-ligand octahedral, coordinating system. Extensive intermolecular hydrophobic interactions, occur at the interfacial recognition site of each neurotoxin molecule, which provides an insight into phospholipase A(2)-membrane interactions., This hydrophobic interaction-induced molecular association along the, interfacial recognition site suggests a self-protection mechanism of, agkistrodotoxin.
<StructureSection load='1bjj' size='340' side='right'caption='[[1bjj]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1bjj]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Gloydius_halys Gloydius halys]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BJJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BJJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bjj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bjj OCA], [https://pdbe.org/1bjj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bjj RCSB], [https://www.ebi.ac.uk/pdbsum/1bjj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bjj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PA2N_GLOHA PA2N_GLOHA] Snake venom phospholipase A2 (PLA2) that inhibits neuromuscular transmission by blocking acetylcholine release from the nerve termini. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.<ref>PMID:15032748</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/1bjj_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bjj ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structure of agkistrodotoxin crystallized under basic conditions has been determined at 2.8 A resolution by the molecular-replacement technique and refined to a crystallographic R factor of 0.194 and a free R factor of 0.260 with good stereochemistry. The molecular packing in the crystal differs from other PLA(2)s. The six molecules in the asymmetric unit form three dimers linked by Ca(2+) ions in a near-perfect six-ligand octahedral coordinating system. Extensive intermolecular hydrophobic interactions occur at the interfacial recognition site of each neurotoxin molecule, which provides an insight into phospholipase A(2)-membrane interactions. This hydrophobic interaction-induced molecular association along the interfacial recognition site suggests a self-protection mechanism of agkistrodotoxin.


==About this Structure==
Structure of agkistrodotoxin in an orthorhombic crystal form with six molecules per asymmetric unit.,Tang L, Zhou YC, Lin ZJ Acta Crystallogr D Biol Crystallogr. 1999 Dec;55(Pt 12):1986-96. PMID:10666574<ref>PMID:10666574</ref>
1BJJ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Gloydius_halys Gloydius halys] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Phospholipase_A(2) Phospholipase A(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.4 3.1.1.4] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BJJ OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure of agkistrodotoxin in an orthorhombic crystal form with six molecules per asymmetric unit., Tang L, Zhou YC, Lin ZJ, Acta Crystallogr D Biol Crystallogr. 1999 Dec;55(Pt 12):1986-96. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10666574 10666574]
</div>
<div class="pdbe-citations 1bjj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Gloydius halys]]
[[Category: Gloydius halys]]
[[Category: Phospholipase A(2)]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Lin Z]]
[[Category: Lin, Z.]]
[[Category: Tang L]]
[[Category: Tang, L.]]
[[Category: Zhou Y]]
[[Category: Zhou, Y.]]
[[Category: CA]]
[[Category: hydrolase]]
[[Category: phospholipase a2]]
[[Category: presynaptic neurotoxin]]
 
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Latest revision as of 11:02, 2 August 2023

AGKISTRODOTOXIN, A PHOSPHOLIPASE A2-TYPE PRESYNAPTIC NEUROTOXIN FROM AGKISTRODON HALYS PALLAS

1bjj, resolution 2.80Å

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