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New page: left|200px<br /><applet load="1bm8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bm8, resolution 1.71Å" /> '''DNA-BINDING DOMAIN O...
 
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[[Image:1bm8.jpg|left|200px]]<br /><applet load="1bm8" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1bm8, resolution 1.71&Aring;" />
'''DNA-BINDING DOMAIN OF MBP1'''<br />


==Overview==
==DNA-BINDING DOMAIN OF MBP1==
BACKGROUND: During the cell cycle, cells progress through four distinct, phases, G1, S, G2 and M; transcriptional controls play an important role, at the transition between these phases. MCB-binding factor (MBF), a, transcription factor from budding yeast, binds to the so-called MCB (MluI, cell-cycle box) elements found in the promoters of many DNA synthesis, genes, and activates the transcription of those at the G1--&gt;S phase, transition. MBF is comprised of two proteins, Mbp1 and Swi6. RESULTS: The, three-dimensional structure of the N-terminal DNA-binding domain of Mbp1, has been determined by multiwavelength anomalous diffraction from crystals, of the selenomethionyl variant of the protein. The structure is composed, of a six-stranded beta sheet interspersed with two pairs of alpha helices., The most conserved core region among Mbp1-related transcription factors, folds into a central helix-turn-helix motif with a short N-terminal beta, strand and a C-terminal beta hairpin. CONCLUSIONS: Despite little sequence, similarity, the structure within the core region of the Mbp1 N-terminal, domain exhibits a similar fold to that of the DNA-binding domains of other, proteins, such as hepatocyte nuclear factor-3gamma and histone H5 from, eukaryotes, and the prokaryotic catabolite gene activator. However, the, structure outside the core region defines Mbp1 as a larger entity with, substructures that stabilize and display the helix-turn-helix motif.
<StructureSection load='1bm8' size='340' side='right'caption='[[1bm8]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1bm8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BM8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BM8 FirstGlance]. <br>
1BM8 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BM8 OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.71&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bm8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bm8 OCA], [https://pdbe.org/1bm8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bm8 RCSB], [https://www.ebi.ac.uk/pdbsum/1bm8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bm8 ProSAT]</span></td></tr>
==Reference==
</table>
Crystal structure of the DNA-binding domain of Mbp1, a transcription factor important in cell-cycle control of DNA synthesis., Xu RM, Koch C, Liu Y, Horton JR, Knapp D, Nasmyth K, Cheng X, Structure. 1997 Mar 15;5(3):349-58. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9083114 9083114]
== Function ==
[https://www.uniprot.org/uniprot/MBP1_YEAST MBP1_YEAST] Binds to MCB elements (Mlu I cell cycle box) found in the promoter of most DNA synthesis genes. Transcriptional activation by MBF has an important role in the transition from G1 to S phase. It may have a dual role in that it behaves as an activator of transcription at the G1-S boundary and as a repressor during other stages of the cell cycle.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bm/1bm8_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bm8 ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Single protein]]
[[Category: Cheng X]]
[[Category: Cheng, X.]]
[[Category: Horton JR]]
[[Category: Horton, J.R.]]
[[Category: Knapp D]]
[[Category: Knapp, D.]]
[[Category: Koch C]]
[[Category: Koch, C.]]
[[Category: Liu Y]]
[[Category: Liu, Y.]]
[[Category: Nasmyth K]]
[[Category: Nasmyth, K.]]
[[Category: Xu R-M]]
[[Category: Xu, R.M.]]
[[Category: cell cycle]]
[[Category: cyclins]]
[[Category: dna synthesis]]
[[Category: helix-turn-helix dna-binding domain]]
[[Category: multiwavelength anomalous diffraction]]
[[Category: transcription factor]]
 
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