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New page: left|200px<br /><applet load="1bnz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bnz, resolution 2.0Å" /> '''SSO7D HYPERTHERMOPHIL...
 
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[[Image:1bnz.gif|left|200px]]<br /><applet load="1bnz" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1bnz, resolution 2.0&Aring;" />
'''SSO7D HYPERTHERMOPHILE PROTEIN/DNA COMPLEX'''<br />


==Overview==
==SSO7D HYPERTHERMOPHILE PROTEIN/DNA COMPLEX==
Sso7d and Sac7d are two small (approximately 7,000 Mr), but abundant, chromosomal proteins from the hyperthermophilic archaeabacteria Sulfolobus, solfataricus and S. acidocaldarius respectively. These proteins have high, thermal, acid and chemical stability. They bind DNA without marked, sequence preference and increase the Tm of DNA by approximately 40 degrees, C. Sso7d in complex with GTAATTAC and GCGT(iU)CGC + GCGAACGC was, crystallized in different crystal lattices and the crystal structures were, solved at high resolution. Sso7d binds in the minor groove of DNA and, causes a single-step sharp kink in DNA (approximately 60 degrees) by the, intercalation of the hydrophobic side chains of Val 26 and Met 29. The, intercalation sites are different in the two complexes. Observations of, this novel DNA binding mode in three independent crystal lattices indicate, that it is not a function of crystal packing.
<StructureSection load='1bnz' size='340' side='right'caption='[[1bnz]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1bnz]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BNZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BNZ FirstGlance]. <br>
1BNZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BNZ OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
 
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bnz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bnz OCA], [https://pdbe.org/1bnz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bnz RCSB], [https://www.ebi.ac.uk/pdbsum/1bnz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bnz ProSAT]</span></td></tr>
==Reference==
</table>
The crystal structure of the hyperthermophile chromosomal protein Sso7d bound to DNA., Gao YG, Su SY, Robinson H, Padmanabhan S, Lim L, McCrary BS, Edmondson SP, Shriver JW, Wang AH, Nat Struct Biol. 1998 Sep;5(9):782-6. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9731772 9731772]
== Function ==
[[Category: Single protein]]
[https://www.uniprot.org/uniprot/DN7D_SACS2 DN7D_SACS2] Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature (By similarity). Stimulates the Holliday junction cleavage activity of Hjc (PubMed:11709558).[UniProtKB:P61990]<ref>PMID:11709558</ref>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Sulfolobus acidocaldarius]]
[[Category: Sulfolobus acidocaldarius]]
[[Category: Edmondos, S.P.]]
[[Category: Edmondos SP]]
[[Category: Gao, Y.G.]]
[[Category: Gao Y-G]]
[[Category: Lim, L.]]
[[Category: Lim L]]
[[Category: Mccrary, B.S.]]
[[Category: Mccrary BS]]
[[Category: Padmanabhan, S.]]
[[Category: Padmanabhan S]]
[[Category: Robinson, H.]]
[[Category: Robinson H]]
[[Category: Shrive, J.W.]]
[[Category: Shrive JW]]
[[Category: Su, S.Y.]]
[[Category: Su S-Y]]
[[Category: Wang, A.H.J.]]
[[Category: Wang AH-J]]
[[Category: protein-dna interaction]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 11:47:01 2007''

Latest revision as of 06:38, 7 February 2024

SSO7D HYPERTHERMOPHILE PROTEIN/DNA COMPLEX

1bnz, resolution 2.00Å

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