1bos: Difference between revisions
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New page: left|200px<br /><applet load="1bos" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bos, resolution 2.8Å" /> '''SHIGA-LIKE TOXIN COMP... |
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== | ==SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR== | ||
Shiga-like toxin I (SLT-I) is a virulence factor of Escherichia coli | <StructureSection load='1bos' size='340' side='right'caption='[[1bos]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[1bos]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_H-19B Enterobacteria phage H-19B] and [https://en.wikipedia.org/wiki/Phage_h30 Phage h30]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BOS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BOS FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLA:ALPHA+D-GALACTOSE'>GLA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bos FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bos OCA], [https://pdbe.org/1bos PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bos RCSB], [https://www.ebi.ac.uk/pdbsum/1bos PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bos ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/STXB_BPH19 STXB_BPH19] The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli. | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/1bos_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1bos ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Shiga-like toxin I (SLT-I) is a virulence factor of Escherichia coli strains that cause disease in humans. Like other members of the Shiga toxin family, it consists of an enzymatic (A) subunit and five copies of a binding subunit (the B-pentamer). The B-pentamer binds to a specific glycolipid, globotriaosylceramide (Gb3), on the surface of target cells and thereby plays a crucial role in the entry of the toxin. Here we present the crystal structure at 2.8 A resolution of the SLT-I B-pentamer complexed with an analogue of the Gb3 trisaccharide. The structure reveals a surprising density of binding sites, with three trisaccharide molecules bound to each B-subunit monomer of 69 residues. All 15 trisaccharides bind to one side of the B-pentamer, providing further evidence that this side faces the cell membrane. The structural model is consistent with data from site-directed mutagenesis and binding of carbohydrate analogues, and allows the rational design of therapeutic Gb3 analogues that block the attachment of toxin to cells. | |||
Structure of the shiga-like toxin I B-pentamer complexed with an analogue of its receptor Gb3.,Ling H, Boodhoo A, Hazes B, Cummings MD, Armstrong GD, Brunton JL, Read RJ Biochemistry. 1998 Feb 17;37(7):1777-88. PMID:9485303<ref>PMID:9485303</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 1bos" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Shiga toxin 3D structures|Shiga toxin 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Enterobacteria phage H-19B]] | |||
[[Category: Large Structures]] | |||
[[Category: Phage h30]] | |||
[[Category: Armstrong GD]] | |||
[[Category: Boodhoo A]] | |||
[[Category: Brunton JL]] | |||
[[Category: Cummings MD]] | |||
[[Category: Hazes B]] | |||
[[Category: Ling H]] | |||
[[Category: Read RJ]] | |||
Latest revision as of 08:21, 6 November 2024
SHIGA-LIKE TOXIN COMPLEXED WITH ITS RECEPTOR
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