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New page: left|200px<br /> <applet load="1hjk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1hjk, resolution 2.3Å" /> '''ALKALINE PHOSPHATASE...
 
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[[Image:1hjk.gif|left|200px]]<br />
<applet load="1hjk" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1hjk, resolution 2.3&Aring;" />
'''ALKALINE PHOSPHATASE MUTANT H331Q'''<br />


==Overview==
==ALKALINE PHOSPHATASE MUTANT H331Q==
Using a mutant version of E. coli alkaline phosphatase, we succeeded in, trapping and determining the structure of the phospho-enzyme intermediate., The X-ray structure also revealed the catalytic water molecule, bound to, one of the active site zinc ions, positioned ideally for the apical attack, necessary for the hydrolysis of the intermediate.
<StructureSection load='1hjk' size='340' side='right'caption='[[1hjk]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1hjk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1HJK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1HJK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1hjk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1hjk OCA], [https://pdbe.org/1hjk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1hjk RCSB], [https://www.ebi.ac.uk/pdbsum/1hjk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1hjk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PPB_ECOLI PPB_ECOLI]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/hj/1hjk_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1hjk ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1HJK is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]] with ZN, MG and SO4 as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1HJK OCA]].
*[[Alkaline phosphatase 3D structures|Alkaline phosphatase 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
Trapping and visualization of a covalent enzyme-phosphate intermediate., Murphy JE, Stec B, Ma L, Kantrowitz ER, Nat Struct Biol. 1997 Aug;4(8):618-22. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9253408 9253408]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Kantrowitz, E.R.]]
[[Category: Kantrowitz ER]]
[[Category: Ma, L.]]
[[Category: Ma L]]
[[Category: Murphy, J.E.]]
[[Category: Murphy JE]]
[[Category: Stec, B.]]
[[Category: Stec B]]
[[Category: MG]]
[[Category: SO4]]
[[Category: ZN]]
[[Category: alcohol acceptor)]]
[[Category: alkaline phosphatase]]
[[Category: hydrolase]]
[[Category: phosphoric monoester]]
[[Category: transferase(phospho]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 19:28:31 2007''

Latest revision as of 11:32, 27 March 2024

ALKALINE PHOSPHATASE MUTANT H331Q

1hjk, resolution 2.30Å

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