2r89: Difference between revisions

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New page: '''Unreleased structure''' The entry 2r89 is ON HOLD until Paper Publication Authors: Hearn, E.M., Patel, D.R., van den Berg, B. Description: Crystal structure of the long-chain fatty ...
 
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'''Unreleased structure'''


The entry 2r89 is ON HOLD  until Paper Publication
==Crystal structure of the long-chain fatty acid transporter FadL mutant delta N3==
<StructureSection load='2r89' size='340' side='right'caption='[[2r89]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[2r89]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2R89 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2R89 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2r89 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2r89 OCA], [https://pdbe.org/2r89 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2r89 RCSB], [https://www.ebi.ac.uk/pdbsum/2r89 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2r89 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FADL_ECOLI FADL_ECOLI] Involved in translocation of long-chain fatty acids across the outer membrane. It is a receptor for the bacteriophage T2. FadL may form a specific channel.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r8/2r89_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2r89 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ligand-gated channels, in which a substrate transport pathway is formed as a result of the binding of a small-molecule chemical messenger, constitute a diverse class of membrane proteins with important functions in prokaryotic and eukaryotic organisms. Despite their widespread nature, no ligand-gated channels have yet been found within the outer membrane (OM) of Gram-negative bacteria. Here we show, using in vivo transport assays, intrinsic tryptophan fluorescence and X-ray crystallography, that high-affinity (submicromolar) substrate binding to the OM long-chain fatty acid transporter FadL from Escherichia coli causes conformational changes in the N terminus that open up a channel for substrate diffusion. The OM long-chain fatty acid transporter FadL from E. coli is a unique paradigm for OM diffusion-driven transport, in which ligand gating within a beta-barrel membrane protein is a prerequisite for channel formation.


Authors: Hearn, E.M., Patel, D.R., van den Berg, B.
Ligand-gated diffusion across the bacterial outer membrane.,Lepore BW, Indic M, Pham H, Hearn EM, Patel DR, van den Berg B Proc Natl Acad Sci U S A. 2011 May 18. PMID:21593406<ref>PMID:21593406</ref>


Description: Crystal structure of the long-chain fatty acid transporter FadL mutant delta N3
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
 
</div>
 
<div class="pdbe-citations 2r89" style="background-color:#fffaf0;"></div>
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 08:47:57 2008''
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Large Structures]]
[[Category: Hearn EM]]
[[Category: Indic M]]
[[Category: Lepore BW]]
[[Category: Patel DR]]
[[Category: Van den Berg B]]
Retrieved from "https://proteopedia.org/2r89"