2zds: Difference between revisions
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New page: '''Unreleased structure''' The entry 2zds is ON HOLD until Nov 27 2009 Authors: Begum, P., Sakai, N., Hayashi, T., Tamura, T., Watanabe, N., Yao, M., Tanaka, I. Description: Crystal St... |
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The | ==Crystal Structure of SCO6571 from Streptomyces coelicolor A3(2)== | ||
<StructureSection load='2zds' size='340' side='right'caption='[[2zds]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2zds]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinomyces_coelicolor"_(muller_1908)_lieske_1921 "actinomyces coelicolor" (muller 1908) lieske 1921]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZDS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZDS FirstGlance]. <br> | |||
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">sco6571 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 "Actinomyces coelicolor" (Muller 1908) Lieske 1921])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zds FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zds OCA], [https://pdbe.org/2zds PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zds RCSB], [https://www.ebi.ac.uk/pdbsum/2zds PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zds ProSAT]</span></td></tr> | |||
</table> | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zd/2zds_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zds ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
SCO6571 protein from Streptomyces coelicolor A3(2) was overexpressed and purified using Rhodococcus erythropolis as an expressing host. Crystals of selenomethionine-substituted SCO6571 have been obtained by vapor diffusion method. SCO6571 crystals diffract to 2.3 A and were found to belong to the orthorhombic space group P2(1)2(1)2(1) with unit cell parameters a = 84.5, b = 171.6, c = 184.8 A. Six molecules in the asymmetric unit give a crystal volume per protein mass (V(M)) of 2.97 A (3) Da(-1) and solvent content of 58.6 %. The structure was solved by the single wavelength anomalous diffraction (SAD) method. SCO6571 is a TIM-barrel fold protein that assembles into a hexameric molecule with D(3) symmetry. | |||
Crystal structure of SCO6571 from Streptomyces coelicolor A3(2).,Begum P, Sakai N, Hayashi T, Gao YG, Tamura T, Watanabe N, Yao M, Tanaka I Protein Pept Lett. 2008;15(7):709-12. PMID:18782066<ref>PMID:18782066</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2zds" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Begum, P]] | |||
[[Category: Gao, Y G]] | |||
[[Category: Sakai, N]] | |||
[[Category: Tanaka, I]] | |||
[[Category: Watanabe, N]] | |||
[[Category: Yao, M]] | |||
[[Category: Dna binding protein]] | |||
[[Category: Dna-binding]] | |||
[[Category: National project on protein structural and functional analyse]] | |||
[[Category: Nppsfa]] | |||
[[Category: Structural genomic]] | |||
[[Category: Tim-barrel fold]] | |||
