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| [[Image:3c68.jpg|left|200px]] | | #REDIRECT [[3w7t]] This PDB entry is obsolete and replaced by 3w7t |
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| {{STRUCTURE_3c68| PDB=3c68 | SCENE= }}
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| '''Escherichia coli K12 YgjK in a complexed with mannose'''
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| ==Overview==
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| Processing alpha-glucosidase I, which is classified into glycosyl hydrolase (GH) family 63, hydrolyzes an oligosaccharide precursor of eukaryotic N-linked glycoproteins. Recently, many bacteria have been reported to possess genes for proteins that are homologous to the GH family 63 glucosidases. In this paper, Escherichia coli K12 YgjK protein, a member of GH family 63, was overexpressed, purified and crystallized using the vapour-diffusion method. Diffraction data were collected to 1.8 A resolution and the crystal was found to belong to the monoclinic space group P2(1), with unit-cell parameters a = 88.5, b = 137.1, c = 60.9 A, beta = 98.1 degrees. The V(M) value was determined to be 2.1 A(3) Da(-1), which corresponds to the presence of two protein molecules in the asymmetric unit.
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| ==About this Structure==
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| 3C68 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C68 OCA].
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| ==Reference==
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| Crystallization and preliminary X-ray analysis of Escherichia coli K12 YgjK protein, a member of glycosyl hydrolase family 63., Tonozuka T, Uechi A, Mizuno M, Ichikawa K, Nishikawa A, Sakano Y, Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1284-5. Epub 2004, Jun 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15213393 15213393]
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| [[Category: Escherichia coli]]
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| [[Category: Single protein]]
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| [[Category: Kamitori, S.]]
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| [[Category: Kurakata, Y.]]
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| [[Category: Nishikawa, A.]]
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| [[Category: Sakano, Y.]]
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| [[Category: Tonozuka, T.]]
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| [[Category: Uechi, A.]]
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| [[Category: Yoshida, H.]]
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| [[Category: Alpha/alpha barrel]]
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| [[Category: Gh63]]
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| [[Category: Hydrolase]]
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| [[Category: Processing alpha-glucosidase]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:48:33 2008''
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This PDB entry is obsolete and replaced by 3w7t
