2qxf: Difference between revisions

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[[Image:2qxf.jpg|left|200px]]


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==Product bound structure of exonuclease I at 1.5 angstrom resolution==
The line below this paragraph, containing "STRUCTURE_2qxf", creates the "Structure Box" on the page.
<StructureSection load='2qxf' size='340' side='right'caption='[[2qxf]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2qxf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QXF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2QXF FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
-->
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TMP:THYMIDINE-5-PHOSPHATE'>TMP</scene></td></tr>
{{STRUCTURE_2qxf| PDB=2qxf |  SCENE= }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2qxf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qxf OCA], [https://pdbe.org/2qxf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2qxf RCSB], [https://www.ebi.ac.uk/pdbsum/2qxf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2qxf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/EX1_ECOLI EX1_ECOLI] Also functions as a DNA deoxyribophosphodiesterase that releases deoxyribose-phosphate moieties following the cleavage DNA at an apurinic/apyrimidinic (AP) site by either an AP endonuclease AP lyase.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qx/2qxf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2qxf ConSurf].
<div style="clear:both"></div>


'''Product bound structure of exonuclease I at 1.5 angstrom resolution'''
==See Also==
 
*[[Exonuclease 3D structures|Exonuclease 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
In Escherichia coli, exonuclease I (ExoI) is a monomeric processive 3'-5' exonuclease that degrades single-stranded DNA. The enzyme has been implicated as primarily being involved in repairing frameshift mutations. The structure of the enzyme has previously been determined in a hexagonal space group at 2.4 A resolution. Here, the structure of ExoI in complex with a nucleotide product, thymidine 5'-monophosphate, is described in an orthorhombic space group at 1.5 A resolution. This new high-resolution structure provides some insight into the interactions involved in binding a nucleotide product. The conserved active site contains a unique metal-binding position when compared with orthologous sites in the Klenow fragment, T4 DNA polymerase and dnaQ. This unique difference is proposed to be a consequence of the repositioning of an important histidine, His181, away from the active site.
[[Category: Escherichia coli K-12]]
 
[[Category: Large Structures]]
==About this Structure==
[[Category: Busam RD]]
2QXF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_k12 Escherichia coli k12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2QXF OCA].
 
==Reference==
Structure of Escherichia coli exonuclease I in complex with thymidine 5'-monophosphate., Busam RD, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):206-10. Epub 2008, Jan 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18219121 18219121]
[[Category: Escherichia coli k12]]
[[Category: Exodeoxyribonuclease I]]
[[Category: Single protein]]
[[Category: Busam, R D.]]
[[Category: Alpha-beta domain]]
[[Category: Dna damage]]
[[Category: Dna repair]]
[[Category: Dnaq superfamily]]
[[Category: Exonuclease]]
[[Category: Hydrolase]]
[[Category: Nuclease]]
[[Category: Product bound structure]]
[[Category: Sh3-like domain]]
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 11 10:56:07 2008''
Retrieved from "https://proteopedia.org/2qxf"