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New page: left|200px<br /><applet load="1cei" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cei, resolution 1.8Å" /> '''STRUCTURE DETERMINATI...
 
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[[Image:1cei.gif|left|200px]]<br /><applet load="1cei" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1cei, resolution 1.8&Aring;" />
'''STRUCTURE DETERMINATION OF THE COLICIN E7 IMMUNITY PROTEIN (IMME7) THAT BINDS SPECIFICALLY TO THE DNASE-TYPE COLICIN E7 AND INHIBITS ITS BACTERIOCIDAL ACTIVITY'''<br />


==Overview==
==STRUCTURE DETERMINATION OF THE COLICIN E7 IMMUNITY PROTEIN (IMME7) THAT BINDS SPECIFICALLY TO THE DNASE-TYPE COLICIN E7 AND INHIBITS ITS BACTERIOCIDAL ACTIVITY==
The immunity protein of colicin E7 (ImmE7) can bind specifically to the, DNase-type colicin E7 and inhibit its bactericidal activity. Here we, report the 1.8-angstrom crystal structure of the ImmE7 protein. This is, the first x-ray structure determined in the superfamily of colicin, immunity proteins. The ImmE7 protein consists of four antiparallel, alpha-helices, folded in a topology similar to the architecture of a, four-helix bundle structure. A region rich in acidic residues is, identified. This negatively charged area has the greatest variability, within the family of DNase-type immunity proteins; thus, it seems likely, that this area is involved in specific binding to colicin. Based on, structural, genetic, and kinetic data, we suggest that all the DNase-type, immunity proteins, as well as colicins, share a "homologous-structural, framework" and that specific interaction between a colicin and its cognate, immunity protein relies upon how well these two proteins' charged residues, match on the interaction surface, thus leading to specific immunity of the, colicin.
<StructureSection load='1cei' size='340' side='right'caption='[[1cei]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1cei]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CEI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CEI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cei FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cei OCA], [https://pdbe.org/1cei PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cei RCSB], [https://www.ebi.ac.uk/pdbsum/1cei PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cei ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IMM7_ECOLX IMM7_ECOLX] This protein is able to protect a cell, which harbors the plasmid ColE7 encoding colicin E7, against colicin E7, it binds specifically to the DNase-type colicin and inhibits its bactericidal activity. Dimeric ImmE7 may possess a RNase activity that cleaves its own mRNA at a specific site and thus autoregulates translational expression of the downstream ceiE7 gene as well as degradation of the upstream ceaE7 mRNA.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ce/1cei_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cei ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1CEI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CEI OCA].
*[[Colicin immunity protein 3D structures|Colicin immunity protein 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The crystal structure of the immunity protein of colicin E7 suggests a possible colicin-interacting surface., Chak KF, Safo MK, Ku WY, Hsieh SY, Yuan HS, Proc Natl Acad Sci U S A. 1996 Jun 25;93(13):6437-42. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8692833 8692833]
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Chak, K.F.]]
[[Category: Chak K-F]]
[[Category: Hsieh, S.Y.]]
[[Category: Hsieh S-Y]]
[[Category: Ku, W.Y.]]
[[Category: Ku W-Y]]
[[Category: Safo, M.K.]]
[[Category: Safo MK]]
[[Category: Yuan, H.S.]]
[[Category: Yuan HS]]
[[Category: immunity protein]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:23:10 2007''

Latest revision as of 06:42, 7 February 2024

STRUCTURE DETERMINATION OF THE COLICIN E7 IMMUNITY PROTEIN (IMME7) THAT BINDS SPECIFICALLY TO THE DNASE-TYPE COLICIN E7 AND INHIBITS ITS BACTERIOCIDAL ACTIVITY

1cei, resolution 1.80Å

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