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New page: left|200px<br /><applet load="1cj5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cj5" /> '''BOVINE BETA-LACTOGLOBULIN A'''<br /> ==Over...
 
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[[Image:1cj5.gif|left|200px]]<br /><applet load="1cj5" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1cj5" />
'''BOVINE BETA-LACTOGLOBULIN A'''<br />


==Overview==
==BOVINE BETA-LACTOGLOBULIN A==
Using heteronuclear NMR spectroscopy, we studied the solution structure, and dynamics of bovine beta-lactoglobulin A at pH 2.0 and 45 degrees C, where the protein exists as a monomeric native state. The monomeric NMR, structure, comprising an eight-stranded continuous antiparallel, beta-barrel and one major alpha-helix, is similar to the X-ray dimeric, structure obtained at pH 6.2, including betaI-strand that forms the dimer, interface and loop EF that serves as a lid of the interior hydrophobic, hole. [1H]-15N NOE revealed that betaF, betaG, and betaH strands buried, under the major alpha-helix are rigid on a pico- to nanosecond time scale, and also emphasized rapid fluctuations of loops and the N- and C-terminal, regions.
<StructureSection load='1cj5' size='340' side='right'caption='[[1cj5]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1cj5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CJ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CJ5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cj5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cj5 OCA], [https://pdbe.org/1cj5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cj5 RCSB], [https://www.ebi.ac.uk/pdbsum/1cj5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cj5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LACB_BOVIN LACB_BOVIN] Primary component of whey, it binds retinol and is probably involved in the transport of that molecule.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cj/1cj5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cj5 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Using heteronuclear NMR spectroscopy, we studied the solution structure and dynamics of bovine beta-lactoglobulin A at pH 2.0 and 45 degrees C, where the protein exists as a monomeric native state. The monomeric NMR structure, comprising an eight-stranded continuous antiparallel beta-barrel and one major alpha-helix, is similar to the X-ray dimeric structure obtained at pH 6.2, including betaI-strand that forms the dimer interface and loop EF that serves as a lid of the interior hydrophobic hole. [1H]-15N NOE revealed that betaF, betaG, and betaH strands buried under the major alpha-helix are rigid on a pico- to nanosecond time scale and also emphasized rapid fluctuations of loops and the N- and C-terminal regions.


==About this Structure==
Solution structure and dynamics of bovine beta-lactoglobulin A.,Kuwata K, Hoshino M, Forge V, Era S, Batt CA, Goto Y Protein Sci. 1999 Nov;8(11):2541-5. PMID:10595563<ref>PMID:10595563</ref>
1CJ5 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CJ5 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Solution structure and dynamics of bovine beta-lactoglobulin A., Kuwata K, Hoshino M, Forge V, Era S, Batt CA, Goto Y, Protein Sci. 1999 Nov;8(11):2541-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10595563 10595563]
</div>
<div class="pdbe-citations 1cj5" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Beta-lactoglobulin 3D structures|Beta-lactoglobulin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Batt, C.A.]]
[[Category: Batt CA]]
[[Category: Era, S.]]
[[Category: Era S]]
[[Category: Forge, V.]]
[[Category: Forge V]]
[[Category: Goto, Y.]]
[[Category: Goto Y]]
[[Category: Hoshino, M.]]
[[Category: Hoshino M]]
[[Category: Kuwata, K.]]
[[Category: Kuwata K]]
[[Category: beta-lactoglobulin a]]
[[Category: dynamics]]
 
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