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New page: left|200px<br /><applet load="1cm9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cm9, resolution 2.1Å" /> '''CRYSTAL STRUCTURE OF ...
 
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[[Image:1cm9.gif|left|200px]]<br /><applet load="1cm9" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1cm9, resolution 2.1&Aring;" />
'''CRYSTAL STRUCTURE OF VIRAL MACROPHAGE INFLAMMATORY PROTEIN-II'''<br />


==Overview==
==CRYSTAL STRUCTURE OF VIRAL MACROPHAGE INFLAMMATORY PROTEIN-II==
Herpesvirus-8 macrophage inflammatory protein-II (vMIP-II) binds a, uniquely wide spectrum of chemokine receptors. We report the X-ray, structure of vMIP-II determined to 2.1 A resolution. Like RANTES, vMIP-II, crystallizes as a dimer and displays the conventional chemokine tertiary, fold. We have compared the surface topology and electrostatic potential of, vMIP-II to those of eotaxin-1, RANTES, and MCP-3, three CCR3 physiological, agonists with known three-dimensional structures. Surface epitopes, identified on RANTES to be involved in binding to CCR3 are mimicked on the, eotaxin-1 and MCP-3 surface. However, the surface topology of vMIP-II in, these regions is markedly different. The results presented here indicate, that the structural basis for interaction with the chemokine receptor CCR3, by vMIP-II is different from that for the physiological agonists, eotaxin-1, RANTES, and MCP-3. These differences on vMIP-II may be a, consequence of its broad-range receptor recognition capabilities.
<StructureSection load='1cm9' size='340' side='right'caption='[[1cm9]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1cm9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human_gammaherpesvirus_8 Human gammaherpesvirus 8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CM9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CM9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cm9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cm9 OCA], [https://pdbe.org/1cm9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cm9 RCSB], [https://www.ebi.ac.uk/pdbsum/1cm9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cm9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/VMI2_HHV8P VMI2_HHV8P] Blocks infection by several different human immunodeficiency virus type 1 (HIV-1) strains. This occurs because vMIP-II binds to a wide range of chemokine receptors. May form part of the response to host defenses contributing to virus-induced neoplasia and may have relevance to KSHV and HIV-I interactions.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/cm/1cm9_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1cm9 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Herpesvirus-8 macrophage inflammatory protein-II (vMIP-II) binds a uniquely wide spectrum of chemokine receptors. We report the X-ray structure of vMIP-II determined to 2.1 A resolution. Like RANTES, vMIP-II crystallizes as a dimer and displays the conventional chemokine tertiary fold. We have compared the surface topology and electrostatic potential of vMIP-II to those of eotaxin-1, RANTES, and MCP-3, three CCR3 physiological agonists with known three-dimensional structures. Surface epitopes identified on RANTES to be involved in binding to CCR3 are mimicked on the eotaxin-1 and MCP-3 surface. However, the surface topology of vMIP-II in these regions is markedly different. The results presented here indicate that the structural basis for interaction with the chemokine receptor CCR3 by vMIP-II is different from that for the physiological agonists eotaxin-1, RANTES, and MCP-3. These differences on vMIP-II may be a consequence of its broad-range receptor recognition capabilities.


==About this Structure==
Comparison of the structure of vMIP-II with eotaxin-1, RANTES, and MCP-3 suggests a unique mechanism for CCR3 activation.,Fernandez EJ, Wilken J, Thompson DA, Peiper SC, Lolis E Biochemistry. 2000 Oct 24;39(42):12837-44. PMID:11041848<ref>PMID:11041848</ref>
1CM9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_herpesvirus_4 Human herpesvirus 4]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CM9 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Comparison of the structure of vMIP-II with eotaxin-1, RANTES, and MCP-3 suggests a unique mechanism for CCR3 activation., Fernandez EJ, Wilken J, Thompson DA, Peiper SC, Lolis E, Biochemistry. 2000 Oct 24;39(42):12837-44. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11041848 11041848]
</div>
[[Category: Human herpesvirus 4]]
<div class="pdbe-citations 1cm9" style="background-color:#fffaf0;"></div>
[[Category: Single protein]]
== References ==
[[Category: Fernandez, E.J.]]
<references/>
[[Category: Lolis, E.]]
__TOC__
[[Category: chemokine]]
</StructureSection>
[[Category: herpesvirus-8]]
[[Category: Human gammaherpesvirus 8]]
[[Category: karposi's sarcoma]]
[[Category: Large Structures]]
 
[[Category: Fernandez EJ]]
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:34:35 2007''
[[Category: Lolis E]]
Retrieved from "https://proteopedia.org/1cm9"