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New page: left|200px<br /><applet load="1cz0" size="450" color="white" frame="true" align="right" spinBox="true" caption="1cz0, resolution 2.1Å" /> '''INTRON ENCODED HOMING...
 
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[[Image:1cz0.gif|left|200px]]<br /><applet load="1cz0" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1cz0, resolution 2.1&Aring;" />
'''INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION'''<br />


==Overview==
==INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION==
A novel mechanism of DNA endonucleolytic cleavage has been visualized for, the homing endonuclease I-PpoI by trapping the uncleaved enzyme-substrate, complex and comparing it to the previously visualized product complex., This enzyme employs a unique single metal mechanism. A magnesium ion is, coordinated by an asparagine residue and two DNA oxygen atoms and, stabilizes the phosphoanion transition state and the 3'oxygen leaving, group. A hydrolytic water molecule is activated by a histidine residue for, an in-line attack on the scissile phosphate. A strained, enzyme-substrate-metal complex is formed before cleavage, then relaxed, during the reaction.
<StructureSection load='1cz0' size='340' side='right'caption='[[1cz0]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1cz0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1CZ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1CZ0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1cz0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1cz0 OCA], [https://pdbe.org/1cz0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1cz0 RCSB], [https://www.ebi.ac.uk/pdbsum/1cz0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1cz0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PPO1_PHYPO PPO1_PHYPO] Mediates the homing of a group I intron in the ribosomal DNA. Makes a four-base staggered cut in its ribosomal DNA target sequence.


==About this Structure==
==See Also==
1CZ0 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Physarum_polycephalum Physarum polycephalum] with ZN and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1CZ0 OCA].
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
A novel endonuclease mechanism directly visualized for I-PpoI., Galburt EA, Chevalier B, Tang W, Jurica MS, Flick KE, Monnat RJ Jr, Stoddard BL, Nat Struct Biol. 1999 Dec;6(12):1096-9. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10581547 10581547]
[[Category: Large Structures]]
[[Category: Physarum polycephalum]]
[[Category: Physarum polycephalum]]
[[Category: Single protein]]
[[Category: Chevalier B]]
[[Category: Chevalier, B.]]
[[Category: Flick KE]]
[[Category: Flick, K.E.]]
[[Category: Galburt EA]]
[[Category: Galburt, E.A.]]
[[Category: Jurica MS]]
[[Category: Jurica, M.S.]]
[[Category: Stoddard BL]]
[[Category: Stoddard, B.L.]]
[[Category: NA]]
[[Category: ZN]]
[[Category: beta sheet dna binding]]
[[Category: distorted dna]]
[[Category: his-cys box zinc binding site]]
[[Category: protein-dna complex]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 12:51:46 2007''

Latest revision as of 06:46, 7 February 2024

INTRON ENCODED HOMING ENDONUCLEASE I-PPOI/DNA COMPLEX LACKING CATALYTIC METAL ION

1cz0, resolution 2.10Å

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