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New page: left|200px<br /><applet load="9gaf" size="450" color="white" frame="true" align="right" spinBox="true" caption="9gaf, resolution 1.9Å" /> '''PRECURSOR OF THE W11F...
 
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[[Image:9gaf.gif|left|200px]]<br /><applet load="9gaf" size="450" color="white" frame="true" align="right" spinBox="true"
caption="9gaf, resolution 1.9&Aring;" />
'''PRECURSOR OF THE W11F MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM'''<br />


==Overview==
==PRECURSOR OF THE W11F MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM==
A variety of proteins, including glycosylasparaginase, have recently been, found to activate functions by self-catalyzed peptide bond rearrangements, from single-chain precursors. Here we present the 1.9 A crystal structures, of glycosylasparaginase precursors that are able to autoproteolyze via an, N --&gt; O acyl shift. Several conserved residues are aligned around the, scissile peptide bond that is in a highly strained trans peptide bond, configuration. The structure illustrates how a nucleophilic side chain may, attack the scissile peptide bond at the immediate upstream backbone, carbonyl and provides an understanding of the structural basis for peptide, bond cleavage via an N --&gt; O or N --&gt; S acyl shift that is used by various, groups of intramolecular autoprocessing proteins.
<StructureSection load='9gaf' size='340' side='right'caption='[[9gaf]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[9gaf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9GAF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9GAF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLY:GLYCINE'>GLY</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9gaf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9gaf OCA], [https://pdbe.org/9gaf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9gaf RCSB], [https://www.ebi.ac.uk/pdbsum/9gaf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9gaf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ASPG_ELIMR ASPG_ELIMR] Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins. Requires that the glycosylated asparagine moiety is not substituted on its N-(R1) and C- (R2) terminus.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ga/9gaf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=9gaf ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A variety of proteins, including glycosylasparaginase, have recently been found to activate functions by self-catalyzed peptide bond rearrangements from single-chain precursors. Here we present the 1.9 A crystal structures of glycosylasparaginase precursors that are able to autoproteolyze via an N --&gt; O acyl shift. Several conserved residues are aligned around the scissile peptide bond that is in a highly strained trans peptide bond configuration. The structure illustrates how a nucleophilic side chain may attack the scissile peptide bond at the immediate upstream backbone carbonyl and provides an understanding of the structural basis for peptide bond cleavage via an N --&gt; O or N --&gt; S acyl shift that is used by various groups of intramolecular autoprocessing proteins.


==About this Structure==
Structural insights into the mechanism of intramolecular proteolysis.,Xu Q, Buckley D, Guan C, Guo HC Cell. 1999 Sep 3;98(5):651-61. PMID:10490104<ref>PMID:10490104</ref>
9GAF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Elizabethkingia_meningoseptica Elizabethkingia meningoseptica] with GLY as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.26 3.5.1.26] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=9GAF OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structural insights into the mechanism of intramolecular proteolysis., Xu Q, Buckley D, Guan C, Guo HC, Cell. 1999 Sep 3;98(5):651-61. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10490104 10490104]
</div>
<div class="pdbe-citations 9gaf" style="background-color:#fffaf0;"></div>
 
==See Also==
*[[Asparaginase 3D structures|Asparaginase 3D structures]]
*[[Glycosylasparaginase|Glycosylasparaginase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Elizabethkingia meningoseptica]]
[[Category: Elizabethkingia meningoseptica]]
[[Category: N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Guo H-C]]
[[Category: Guo, H.C.]]
[[Category: Xu Q]]
[[Category: Xu, Q.]]
[[Category: GLY]]
[[Category: autoproteolysis]]
[[Category: glycosylasparaginase]]
[[Category: mutant]]
[[Category: n-terminal nucleophile hydrolase]]
[[Category: precursor]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:02:50 2007''

Latest revision as of 18:20, 20 September 2023

PRECURSOR OF THE W11F MUTANT GLYCOSYLASPARAGINASE FROM FLAVOBACTERIUM MENINGOSEPTICUM

9gaf, resolution 1.90Å

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