1d9c: Difference between revisions

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New page: left|200px<br /><applet load="1d9c" size="450" color="white" frame="true" align="right" spinBox="true" caption="1d9c, resolution 2.0Å" /> '''BOVINE INTERFERON-GAM...
 
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[[Image:1d9c.jpg|left|200px]]<br /><applet load="1d9c" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1d9c, resolution 2.0&Aring;" />
'''BOVINE INTERFERON-GAMMA AT 2.0 ANGSTROMS'''<br />


==Overview==
==BOVINE INTERFERON-GAMMA AT 2.0 ANGSTROMS==
The structure of bovine interferon-gamma (IFN-gamma) was determined by, multiple isomorphous replacement at 2.0 A resolution. Bovine IFN-gamma, crystallizes in two related crystal forms. Crystal form 1 diffracts to 2.9, A resolution and is reproducible and stable to derivatization. Crystal, form 2 diffracts to 2.0 A resolution, but shows significant, non-isomorphism from crystal to crystal. The previously determined, structures of several different species of INF-gamma were either at too, low a resolution [human, 1hig; Ealick et al. (1991), Science, 252, 698-702] or were too inaccurate [bovine, 1rfb; Samudzi &amp; Rubin (1993), Acta Cryst. D49(6), 505-512; rabbit, 2rig; Samudzi et al. (1991), J. Biol., Chem. 266(32), 21791-21797] for the structure to be solved by molecular, replacement. The structure was solved in crystal form 1 using two, derivatives produced by chemically modifying two free cysteine residues, that were introduced by site-directed mutagenesis (Ser30Cys, Asn59Cys)., After model building and refinement, the final R value was 21.8% (R(free), = 30.9%) for all data in the resolution range 8.0-2.9 A. The crystal form, 1 structure was then used as a molecular-replacement model for crystal, form 2 data collected from a flash-cooled crystal. Subsequent model, building and refinement, using all data in the resolution range 15.0-2.0, A, gave an R value of 19.7% and an R(free) of 27.5%. Pairwise comparison, of C(alpha) positions of bovine IFN-gamma (BOV) and the previously, determined 1rfb and 2rig structures indicated some significant differences, in the models (r.m.s.d. values for BOV to 1rfb, 4.3 A; BOV to 2rig, 4.0, A). An assessment of the quality of the structures was made using the, 3D-1D algorithm [Eisenberg et al. (1992), Faraday Discuss. 93, 25-34]. The, resulting statistical scoring indicated that BOV was consistent with, expected criteria for a 2.0 A structure, whereas both 1rfb and 2rig fell, below acceptable criteria.
<StructureSection load='1d9c' size='340' side='right'caption='[[1d9c]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1d9c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D9C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D9C FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d9c OCA], [https://pdbe.org/1d9c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d9c RCSB], [https://www.ebi.ac.uk/pdbsum/1d9c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d9c ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/IFNG_BOVIN IFNG_BOVIN] Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/d9/1d9c_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1d9c ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1D9C is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1D9C OCA].
*[[Interferon 3D structures|Interferon 3D structures]]
 
__TOC__
==Reference==
</StructureSection>
The 2.0 A structure of bovine interferon-gamma; assessment of the structural differences between species., Randal M, Kossiakoff AA, Acta Crystallogr D Biol Crystallogr. 2000 Jan;56(Pt 1):14-24. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10666622 10666622]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Kossiakoff, A.A.]]
[[Category: Kossiakoff AA]]
[[Category: Randal, M.]]
[[Category: Randal M]]
[[Category: helical homodimer]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:05:43 2007''

Latest revision as of 06:50, 7 February 2024

BOVINE INTERFERON-GAMMA AT 2.0 ANGSTROMS

1d9c, resolution 2.00Å

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