1dbr: Difference between revisions

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New page: left|200px<br /><applet load="1dbr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dbr, resolution 2.4Å" /> '''HYPOXANTHINE GUANINE ...
 
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[[Image:1dbr.jpg|left|200px]]<br /><applet load="1dbr" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1dbr, resolution 2.4&Aring;" />
'''HYPOXANTHINE GUANINE XANTHINE'''<br />


==Overview==
==HYPOXANTHINE GUANINE XANTHINE==
Crystal structures of substrate-free and XMP-soaked, hypoxanthine-guanine-xanthine phosphoribosyltransferase (HGXPRTase) of the, opportunistic pathogen Toxoplasma gondii have been determined to 2.4 and, 2.9 A resolution, respectively. HGXPRTase displays the conserved PRTase, fold. In the structure of the enzyme bound to its product, a long flexible, loop (residues 115-126) is located away from the active site. Comparison, to the substrate-free structure reveals a striking relocation of the loop, which is poised to cover the catalytic pocket, thus providing a mechanism, by which the HG(X)PRTases shield their oxocarbonium transition states from, nucleophilic attack by the bulk solvent. The conserved Ser 117-Tyr 118, dipeptide within the loop is brought to the active site, completing the, ensemble of catalytic residues.
<StructureSection load='1dbr' size='340' side='right'caption='[[1dbr]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1dbr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DBR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DBR FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dbr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dbr OCA], [https://pdbe.org/1dbr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dbr RCSB], [https://www.ebi.ac.uk/pdbsum/1dbr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dbr ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/HGXR_TOXGO HGXR_TOXGO] Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to the N(9) of hypoxanthine, guanine or xanthine.<ref>PMID:11188695</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/db/1dbr_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dbr ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1DBR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Toxoplasma_gondii Toxoplasma gondii] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DBR OCA].
*[[Phosphoribosyltransferase 3D structures|Phosphoribosyltransferase 3D structures]]
 
== References ==
==Reference==
<references/>
Crystal structures of Toxoplasma gondii HGXPRTase reveal the catalytic role of a long flexible loop., Schumacher MA, Carter D, Ross DS, Ullman B, Brennan RG, Nat Struct Biol. 1996 Oct;3(10):881-7. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=8836106 8836106]
__TOC__
[[Category: Single protein]]
</StructureSection>
[[Category: Large Structures]]
[[Category: Toxoplasma gondii]]
[[Category: Toxoplasma gondii]]
[[Category: Brennan, R.G.]]
[[Category: Brennan RG]]
[[Category: Carter, D.]]
[[Category: Carter D]]
[[Category: Roos, D.]]
[[Category: Roos D]]
[[Category: Schumacher, M.A.]]
[[Category: Schumacher MA]]
[[Category: Ullman, B.]]
[[Category: Ullman B]]
[[Category: MG]]
[[Category: glycosyltransferase]]
[[Category: purine salvage]]
[[Category: transferase]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:08:41 2007''

Latest revision as of 06:51, 7 February 2024

HYPOXANTHINE GUANINE XANTHINE

1dbr, resolution 2.40Å

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