2flf: Difference between revisions

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[[Image:2flf.png|left|200px]]


<!--
==Crystal structure of l-fuculose-1-phosphate aldolase from Thermus Thermophilus HB8==
The line below this paragraph, containing "STRUCTURE_2flf", creates the "Structure Box" on the page.
<StructureSection load='2flf' size='340' side='right'caption='[[2flf]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2flf]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2FLF FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
-->
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2flf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2flf OCA], [https://pdbe.org/2flf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2flf RCSB], [https://www.ebi.ac.uk/pdbsum/2flf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2flf ProSAT], [https://www.topsan.org/Proteins/RSGI/2flf TOPSAN]</span></td></tr>
{{STRUCTURE_2flf|  PDB=2flf  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q5SHB9_THET8 Q5SHB9_THET8]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/fl/2flf_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2flf ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Fuculose phosphate aldolase catalyzes the reversible cleavage of L-fuculose-1-phosphate to dihydroxyacetone phosphate and L-lactaldehyde. The protein from Thermus thermophilus HB8 is a biological tetramer with a subunit molecular weight of 21 591 Da. Purified FucA has been crystallized using sitting-drop vapour-diffusion and microbatch techniques at 293 K. The crystals belong to space group P4, with unit-cell parameters a = b = 100.94, c = 45.87 A. The presence of a dimer of the enzyme in the asymmetric unit was estimated to give a Matthews coefficient (VM) of 2.7 A3 Da(-1) and a solvent content of 54.2%(v/v). Three-wavelength diffraction MAD data were collected to 2.3 A from zinc-containing crystals. Native diffraction data to 1.9 A resolution have been collected using synchrotron radiation at SPring-8.


'''Crystal structure of l-fuculose-1-phosphate aldolase from Thermus Thermophilus HB8'''
Purification, crystallization and preliminary X-ray crystallographic study of the L-fuculose-1-phosphate aldolase (FucA) from Thermus thermophilus HB8.,Jeyakanthan J, Taka J, Kikuchi A, Kuroishi C, Yutani K, Shiro Y Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Dec 1;61(Pt, 12):1075-7. Epub 2005 Nov 24. PMID:16511238<ref>PMID:16511238</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2flf" style="background-color:#fffaf0;"></div>


{{ABSTRACT_16511238}}
==See Also==
 
*[[Aldolase 3D structures|Aldolase 3D structures]]
==About this Structure==
== References ==
2FLF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus_hb8 Thermus thermophilus hb8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2FLF OCA].
<references/>
[[Category: L-fuculose-phosphate aldolase]]
__TOC__
[[Category: Single protein]]
</StructureSection>
[[Category: Thermus thermophilus hb8]]
[[Category: Large Structures]]
[[Category: Jeyakanthan, J.]]
[[Category: Thermus thermophilus HB8]]
[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
[[Category: Jeyakanthan J]]
[[Category: Shiro, Y.]]
[[Category: Shiro Y]]
[[Category: Yokoyama, S.]]
[[Category: Yokoyama S]]
[[Category: Class ii aldolase]]
[[Category: Fuculose phosphate]]
[[Category: Lyase]]
[[Category: Metal binding]]
[[Category: National project on protein structural and functional analyse]]
[[Category: Nppsfa]]
[[Category: Riken structural genomics/proteomics initiative]]
[[Category: Rsgi]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 18:38:32 2008''

Latest revision as of 08:44, 25 October 2023

Crystal structure of l-fuculose-1-phosphate aldolase from Thermus Thermophilus HB8

2flf, resolution 2.70Å

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