2vt4: Difference between revisions

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==TURKEY BETA1 ADRENERGIC RECEPTOR WITH STABILISING MUTATIONS AND BOUND CYANOPINDOLOL==
The line below this paragraph, containing "STRUCTURE_2vt4", creates the "Structure Box" on the page.
<StructureSection load='2vt4' size='340' side='right'caption='[[2vt4]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
You may change the PDB parameter (which sets the PDB file loaded into the applet)  
== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[2vt4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Meleagris_gallopavo Meleagris gallopavo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VT4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VT4 FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=D10:DECANE'>D10</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=P32:4-{[(2S)-3-(TERT-BUTYLAMINO)-2-HYDROXYPROPYL]OXY}-3H-INDOLE-2-CARBONITRILE'>P32</scene>, <scene name='pdbligand=SOG:2-HYDROXYMETHYL-6-OCTYLSULFANYL-TETRAHYDRO-PYRAN-3,4,5-TRIOL'>SOG</scene></td></tr>
{{STRUCTURE_2vt4|  PDB=2vt4  |  SCENE=  }}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vt4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vt4 OCA], [https://pdbe.org/2vt4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vt4 RCSB], [https://www.ebi.ac.uk/pdbsum/2vt4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vt4 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ADRB1_MELGA ADRB1_MELGA] Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vt/2vt4_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2vt4 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
G-protein-coupled receptors have a major role in transmembrane signalling in most eukaryotes and many are important drug targets. Here we report the 2.7 A resolution crystal structure of a beta(1)-adrenergic receptor in complex with the high-affinity antagonist cyanopindolol. The modified turkey (Meleagris gallopavo) receptor was selected to be in its antagonist conformation and its thermostability improved by earlier limited mutagenesis. The ligand-binding pocket comprises 15 side chains from amino acid residues in 4 transmembrane alpha-helices and extracellular loop 2. This loop defines the entrance of the ligand-binding pocket and is stabilized by two disulphide bonds and a sodium ion. Binding of cyanopindolol to the beta(1)-adrenergic receptor and binding of carazolol to the beta(2)-adrenergic receptor involve similar interactions. A short well-defined helix in cytoplasmic loop 2, not observed in either rhodopsin or the beta(2)-adrenergic receptor, directly interacts by means of a tyrosine with the highly conserved DRY motif at the end of helix 3 that is essential for receptor activation.


'''TURKEY BETA1 ADRENERGIC RECEPTOR WITH STABILISING MUTATIONS AND BOUND CYANOPINDOLOL'''
Structure of a beta1-adrenergic G-protein-coupled receptor.,Warne T, Serrano-Vega MJ, Baker JG, Moukhametzianov R, Edwards PC, Henderson R, Leslie AG, Tate CG, Schertler GF Nature. 2008 Jul 24;454(7203):486-91. Epub 2008 Jun 25. PMID:18594507<ref>PMID:18594507</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2vt4" style="background-color:#fffaf0;"></div>


{{ABSTRACT_18192400}}
==See Also==
 
*[[Adrenergic receptor 3D structures|Adrenergic receptor 3D structures]]
==About this Structure==
== References ==
2VT4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Meleagris_gallopavo Meleagris gallopavo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VT4 OCA].
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Meleagris gallopavo]]
[[Category: Meleagris gallopavo]]
[[Category: Single protein]]
[[Category: Baker JG]]
[[Category: Baker, J G.]]
[[Category: Edwards PC]]
[[Category: Edwards, P C.]]
[[Category: Henderson R]]
[[Category: Henderson, R.]]
[[Category: Leslie AGW]]
[[Category: Leslie, A G.W.]]
[[Category: Moukhametzianov R]]
[[Category: Moukhametzianov, R.]]
[[Category: Schertler GFX]]
[[Category: Schertler, G F.X.]]
[[Category: Serrano-Vega MJ]]
[[Category: Serrano-Vega, M J.]]
[[Category: Tate CG]]
[[Category: Tate, C G.]]
[[Category: Warne A]]
[[Category: Warne, A.]]
[[Category: 7tm receptor]]
[[Category: Antagonist bound form]]
[[Category: G protein coupled receptor]]
[[Category: G-protein coupled receptor]]
[[Category: Glycoprotein]]
[[Category: Gpcr]]
[[Category: Integral membrane protein]]
[[Category: Lipoprotein]]
[[Category: Membrane]]
[[Category: Palmitate]]
[[Category: Phosphoprotein]]
[[Category: Receptor]]
[[Category: Seven-helix receptor]]
[[Category: Thermostabilising point mutation]]
[[Category: Transducer]]
[[Category: Transmembrane]]
 
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Retrieved from "https://proteopedia.org/2vt4"