2z46: Difference between revisions

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~~[[Image:2z46.jpg|left|200px]]~~

~~<!--~~

==Crystal Structure of Native-ORF134==

~~The line below this paragraph, containing "STRUCTURE_2z46", creates the "~~Structure ~~Box" on the page.~~

~~You may change the PDB parameter (which sets the PDB file loaded into the applet)~~

== Structural highlights ==

~~or the SCENE parameter (which sets the initial scene displayed when the page is loaded),~~

<table><tr><td colspan='2'>[[2z46]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_sp._PCC_7002 Synechococcus sp. PCC 7002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z46 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z46 FirstGlance]. <br>

~~or leave the SCENE parameter empty for the default display.~~

</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.97Å</td></tr>

-->

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z46 OCA], [https://pdbe.org/2z46 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z46 RCSB], [https://www.ebi.ac.uk/pdbsum/2z46 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z46 ProSAT]</span></td></tr>

~~{{STRUCTURE_2z46| PDB~~=2z46 ~~| SCENE~~= }}

</table>

== Function ==

'''~~Crystal Structure of Native-ORF134~~'''

[https://www.uniprot.org/uniprot/RBCX_SYNP2 RBCX_SYNP2] An RbcL-specific chaperone. Required for assembly of the RbcL8 core, acting downstream of the major chaperonin (GroEL-GroES). Acts on newly folded RbcL, has a transient dynamic interaction with RbcL and is eventually displaced by RbcS (PubMed:17574029). The central cleft of the RbcX homodimer (RbcX2) binds the C-terminus of an RbcL monomer, stabilizing the C-terminus and probably preventing its reassociation with chaperonin GroEL-ES. At the same time the peripheral region of RbcX2 binds a second RbcL monomer, bridging the RbcL homodimers in the correct orientation. The RbcX2(2)-bound RbcL dimers then assemble into the RbcL8 core (RbcL8-(RbcX2)8). RbcS binding triggers the release of RbcX2 (By similarity). Required for optimal reconstitution of RuBisCO into its RbcL8S8 holoenzyme form upon expression of rbcL-rbcS subunits in E.coli, and probably also in situ. A frameshift mutation that replaces half the protein reduces accumulation of both RbcL and RbcS subunits and halves activity of RuBisCO in situ and in E.coli (PubMed:15564522).[UniProtKB:Q44212]<ref>PMID:15564522</ref> <ref>PMID:17574029</ref>

== Evolutionary Conservation ==

[[Image:Consurf_key_small.gif|200px|right]]

==~~About this Structure~~==

Check<jmol>

~~2Z46 is a~~ [[~~Single protein~~]] structure of sequence from [~~http~~://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z46 OCA].

[[Category: ~~Single protein~~]]

<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z4/2z46_consurf.spt"</scriptWhenChecked>

[[Category: Synechococcus sp.]]

<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>

[[Category: Ashida, H.]]

<text>to colour the structure by Evolutionary Conservation</text>

[[Category: Ihara, K.]]

</jmolCheckbox>

[[Category: Kanai, S.]]

</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z46 ConSurf].

[[Category: Kato, R.]]

[[Category: Miyasaka, H.]]

== References ==

[[Category: Onizuka, T.]]

[[Category: Tanaka, S.]]

__TOC__

[[Category: Tomimoto, Y.]]

</StructureSection>

[[Category: Wakatsuki, S.]]

[[Category: Large Structures]]

[[Category: Yamada, Y.]]

[[Category: Synechococcus sp. PCC 7002]]

[[Category: Yokota, A.]]

[[Category: Ashida H]]

~~[[Category: Chaperone]]~~

[[Category: Ihara K]]

~~[[Category: Helix bundle]]~~

[[Category: Kanai S]]

[[Category: Kato R]]

~~''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 18:54:05 2008''~~

[[Category: Miyasaka H]]

[[Category: Onizuka T]]

[[Category: Tanaka S]]

[[Category: Tomimoto Y]]

[[Category: Wakatsuki S]]

[[Category: Yamada Y]]

[[Category: Yokota A]]

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@@ Line 1: / Line 1: @@
-[[Image:2z46.jpg|left|200px]]
-<!--
+==Crystal Structure of Native-ORF134==
-The line below this paragraph, containing "STRUCTURE_2z46", creates the "Structure Box" on the page.
+<StructureSection load='2z46' size='340' side='right'caption='[[2z46]], [[Resolution|resolution]] 2.97&Aring;' scene=''>
-You may change the PDB parameter (which sets the PDB file loaded into the applet)
+== Structural highlights ==
-or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+<table><tr><td colspan='2'>[[2z46]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechococcus_sp._PCC_7002 Synechococcus sp. PCC 7002]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z46 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z46 FirstGlance]. <br>
-or leave the SCENE parameter empty for the default display.
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.97&#8491;</td></tr>
--->
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z46 OCA], [https://pdbe.org/2z46 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z46 RCSB], [https://www.ebi.ac.uk/pdbsum/2z46 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z46 ProSAT]</span></td></tr>
-{{STRUCTURE_2z46|  PDB=2z46  |  SCENE=  }}
+</table>
+== Function ==
-'''Crystal Structure of Native-ORF134'''
+[https://www.uniprot.org/uniprot/RBCX_SYNP2 RBCX_SYNP2] An RbcL-specific chaperone. Required for assembly of the RbcL8 core, acting downstream of the major chaperonin (GroEL-GroES). Acts on newly folded RbcL, has a transient dynamic interaction with RbcL and is eventually displaced by RbcS (PubMed:17574029). The central cleft of the RbcX homodimer (RbcX2) binds the C-terminus of an RbcL monomer, stabilizing the C-terminus and probably preventing its reassociation with chaperonin GroEL-ES. At the same time the peripheral region of RbcX2 binds a second RbcL monomer, bridging the RbcL homodimers in the correct orientation. The RbcX2(2)-bound RbcL dimers then assemble into the RbcL8 core (RbcL8-(RbcX2)8). RbcS binding triggers the release of RbcX2 (By similarity). Required for optimal reconstitution of RuBisCO into its RbcL8S8 holoenzyme form upon expression of rbcL-rbcS subunits in E.coli, and probably also in situ. A frameshift mutation that replaces half the protein reduces accumulation of both RbcL and RbcS subunits and halves activity of RuBisCO in situ and in E.coli (PubMed:15564522).[UniProtKB:Q44212]<ref>PMID:15564522</ref> <ref>PMID:17574029</ref>
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
-==About this Structure==
+Check<jmol>
-Z46 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Synechococcus_sp. Synechococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z46 OCA].
+  <jmolCheckbox>
-[[Category: Single protein]]
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/z4/2z46_consurf.spt"</scriptWhenChecked>
-[[Category: Synechococcus sp.]]
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-[[Category: Ashida, H.]]
+    <text>to colour the structure by Evolutionary Conservation</text>
-[[Category: Ihara, K.]]
+  </jmolCheckbox>
-[[Category: Kanai, S.]]
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2z46 ConSurf].
-[[Category: Kato, R.]]
+<div style="clear:both"></div>
-[[Category: Miyasaka, H.]]
+== References ==
-[[Category: Onizuka, T.]]
+<references/>
-[[Category: Tanaka, S.]]
+__TOC__
-[[Category: Tomimoto, Y.]]
+</StructureSection>
-[[Category: Wakatsuki, S.]]
+[[Category: Large Structures]]
-[[Category: Yamada, Y.]]
+[[Category: Synechococcus sp. PCC 7002]]
-[[Category: Yokota, A.]]
+[[Category: Ashida H]]
-[[Category: Chaperone]]
+[[Category: Ihara K]]
-[[Category: Helix bundle]]
+[[Category: Kanai S]]
+[[Category: Kato R]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jun 25 18:54:05 2008''
+[[Category: Miyasaka H]]
+[[Category: Onizuka T]]
+[[Category: Tanaka S]]
+[[Category: Tomimoto Y]]
+[[Category: Wakatsuki S]]
+[[Category: Yamada Y]]
+[[Category: Yokota A]]