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New page: left|200px<br /><applet load="1dhr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1dhr, resolution 2.3Å" /> '''CRYSTAL STRUCTURE OF ...
 
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[[Image:1dhr.gif|left|200px]]<br /><applet load="1dhr" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1dhr, resolution 2.3&Aring;" />
'''CRYSTAL STRUCTURE OF RAT LIVER DIHYDROPTERIDINE REDUCTASE'''<br />


==Overview==
==CRYSTAL STRUCTURE OF RAT LIVER DIHYDROPTERIDINE REDUCTASE==
The structure of a binary complex of dihydropteridine reductase [DHPR;, NAD(P)H:6,7-dihydropteridine oxidoreductase, EC 1.6.99.7] with its, cofactor, NADH, has been solved and refined to a final R factor of 15.4%, by using 2.3 A diffraction data. DHPR is an alpha/beta protein with a, Rossmann-type dinucleotide fold for NADH binding. Insertion of an extra, threonine residue in the human enzyme is associated with severe symptoms, of a variant form of phenylketonuria and maps to a tightly linked sequence, of secondary-structural elements near the dimer interface. Dimerization is, mediated by a four-helix bundle motif (two helices from each protomer), having an unusual right-handed twist. DHPR is structurally and, mechanistically distinct from dihydrofolate reductase, appearing to more, closely resemble certain nicotinamide dinucleotide-requiring, flavin-dependent enzymes, such as glutathione reductase.
<StructureSection load='1dhr' size='340' side='right'caption='[[1dhr]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 
== Structural highlights ==
==About this Structure==
<table><tr><td colspan='2'>[[1dhr]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. The August 2015 RCSB PDB [https://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/index.html Molecule of the Month] feature on ''Tetrahydrobiopterin Biosynthesis''  by David Goodsell is [https://dx.doi.org/10.2210/rcsb_pdb/mom_2015_8 10.2210/rcsb_pdb/mom_2015_8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DHR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DHR FirstGlance]. <br>
1DHR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus] with NAD as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/6,7-dihydropteridine_reductase 6,7-dihydropteridine reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.5.1.34 1.5.1.34] Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1DHR OCA].  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
 
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
==Reference==
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dhr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dhr OCA], [https://pdbe.org/1dhr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dhr RCSB], [https://www.ebi.ac.uk/pdbsum/1dhr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dhr ProSAT]</span></td></tr>
Crystal structure of rat liver dihydropteridine reductase., Varughese KI, Skinner MM, Whiteley JM, Matthews DA, Xuong NH, Proc Natl Acad Sci U S A. 1992 Jul 1;89(13):6080-4. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=1631094 1631094]
</table>
[[Category: 6,7-dihydropteridine reductase]]
== Function ==
[https://www.uniprot.org/uniprot/DHPR_RAT DHPR_RAT] The product of this enzyme, tetrahydrobiopterin (BH-4), is an essential cofactor for phenylalanine, tyrosine, and tryptophan hydroxylases.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dh/1dhr_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dhr ConSurf].
<div style="clear:both"></div>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: RCSB PDB Molecule of the Month]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Single protein]]
[[Category: Tetrahydrobiopterin Biosynthesis]]
[[Category: Matthews, D.A.]]
[[Category: Matthews DA]]
[[Category: Skinner, M.M.]]
[[Category: Skinner MM]]
[[Category: Varughese, K.I.]]
[[Category: Varughese KI]]
[[Category: Whiteley, J.M.]]
[[Category: Whiteley JM]]
[[Category: Xuong, N.H.]]
[[Category: Xuong NH]]
[[Category: NAD]]
[[Category: oxidoreductase(acting on nadh or nadph)]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Nov 20 13:17:26 2007''

Latest revision as of 06:53, 7 February 2024

CRYSTAL STRUCTURE OF RAT LIVER DIHYDROPTERIDINE REDUCTASE

1dhr, resolution 2.30Å

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