1h4j: Difference between revisions

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New page: left|200px<br /> <applet load="1h4j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1h4j, resolution 3.0Å" /> '''METHYLOBACTERIUM EXT...
 
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[[Image:1h4j.gif|left|200px]]<br />
<applet load="1h4j" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1h4j, resolution 3.0&Aring;" />
'''METHYLOBACTERIUM EXTORQUENS METHANOL DEHYDROGENASE D303E MUTANT'''<br />


==Overview==
==Methylobacterium extorquens methanol dehydrogenase D303E mutant==
Two proteins specifically involved in methanol oxidation in the, methylotrophic bacterium Methylobacterium extorquens have been modified by, site-directed mutagenesis. Mutation of the proposed active site base, (Asp303) to glutamate in methanol dehydrogenase (MDH) gave an active, enzyme (D303E-MDH) with a greatly reduced affinity for substrate and with, a lower activation energy. Results of kinetic and deuterium isotope, studies showed that the essential mechanism in the mutant protein was, unchanged, and that the step requiring activation by ammonia remained rate, limiting. No spectrally detectable intermediates could be observed during, the reaction. The X-ray structure, determined to 3 A resolution, of, D303E-MDH showed that the position and coordination geometry of the Ca2+, ion in ... [[http://ispc.weizmann.ac.il/pmbin/getpm?11502173 (full description)]]
<StructureSection load='1h4j' size='340' side='right'caption='[[1h4j]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1h4j]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylorubrum_extorquens Methylorubrum extorquens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1H4J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1H4J FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PQQ:PYRROLOQUINOLINE+QUINONE'>PQQ</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1h4j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1h4j OCA], [https://pdbe.org/1h4j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1h4j RCSB], [https://www.ebi.ac.uk/pdbsum/1h4j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1h4j ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DHM1_METEA DHM1_METEA] Catalyzes the oxidation of primary alcohols including methanol.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/h4/1h4j_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1h4j ConSurf].
<div style="clear:both"></div>


==About this Structure==
==See Also==
1H4J is a [[http://en.wikipedia.org/wiki/Protein_complex Protein complex]] structure of sequences from [[http://en.wikipedia.org/wiki/Methylobacterium_extorquens Methylobacterium extorquens]] with CA and PQQ as [[http://en.wikipedia.org/wiki/ligands ligands]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.99.8 1.1.99.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1H4J OCA]].
*[[Methanol dehydrogenase|Methanol dehydrogenase]]
 
__TOC__
==Reference==
</StructureSection>
Site-directed mutagenesis and X-ray crystallography of the PQQ-containing quinoprotein methanol dehydrogenase and its electron acceptor, cytochrome c(L)., Afolabi PR, Mohammed F, Amaratunga K, Majekodunmi O, Dales SL, Gill R, Thompson D, Cooper JB, Wood SP, Goodwin PM, Anthony C, Biochemistry. 2001 Aug 21;40(33):9799-809. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=11502173 11502173]
[[Category: Large Structures]]
[[Category: Methylobacterium extorquens]]
[[Category: Methylorubrum extorquens]]
[[Category: Protein complex]]
[[Category: Afolabi PR]]
[[Category: Afolabi, P.R.]]
[[Category: Anthony C]]
[[Category: Anthony, C.]]
[[Category: Cooper JB]]
[[Category: Cooper, J.B.]]
[[Category: Gill R]]
[[Category: Gill, R.]]
[[Category: Mohammed F]]
[[Category: Mohammed, F.]]
[[Category: Thompson D]]
[[Category: Thompson, D.]]
[[Category: Wood SP]]
[[Category: Wood, S.P.]]
[[Category: CA]]
[[Category: PQQ]]
[[Category: dehydrogenase]]
[[Category: quinoprotein]]
 
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Latest revision as of 11:27, 27 March 2024

Methylobacterium extorquens methanol dehydrogenase D303E mutant

1h4j, resolution 3.00Å

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