1arb: Difference between revisions

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{{Seed}}
[[Image:1arb.png|left|200px]]


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==THE PRIMARY STRUCTURE AND STRUCTURAL CHARACTERISTICS OF ACHROMOBACTER LYTICUS PROTEASE I, A LYSINE-SPECIFIC SERINE PROTEASE==
The line below this paragraph, containing "STRUCTURE_1arb", creates the "Structure Box" on the page.
<StructureSection load='1arb' size='340' side='right'caption='[[1arb]], [[Resolution|resolution]] 1.20&Aring;' scene=''>
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== Structural highlights ==
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
<table><tr><td colspan='2'>[[1arb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Achromobacter_lyticus Achromobacter lyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ARB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ARB FirstGlance]. <br>
or leave the SCENE parameter empty for the default display.
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.2&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1arb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1arb OCA], [https://pdbe.org/1arb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1arb RCSB], [https://www.ebi.ac.uk/pdbsum/1arb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1arb ProSAT]</span></td></tr>
{{STRUCTURE_1arb|  PDB=1arb  |  SCENE=  }}
</table>
== Function ==
[https://www.uniprot.org/uniprot/API_ACHLY API_ACHLY]
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ar/1arb_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1arb ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The complete amino acid sequence of Achromobacter lyticus protease I (EC 3.4.21.50), which specifically hydrolyzes lysyl peptide bonds, has been established. This has been achieved by sequence analysis of the reduced and S-carboxymethylated protease and of peptides obtained by enzymatic digestion with Achromobacter protease I itself and Staphylococcus aureus V8 protease and by chemical cleavage with cyanogen bromide. The protease consists of 268 residues with three disulfide bonds, which have been assigned to Cys6-Cys216, Cys12-Cys80, and Cys36-Cys58. Comparison of the amino acid sequence of Achromobacter protease and other serine proteases of bacterial and mammalian origins has revealed that Achromobacter protease I is a mammalian-type serine protease of which the catalytic triad comprises His57, Asp113, and Ser194. It has also been shown that the protease has 9- and 26-residue extensions of the peptide chain at the N and C termini, respectively, and overall sequence homology is as low as 20% with bovine trypsin. The presence of a disulfide bridge between the N-terminal extension Cys6 and Cys216 close to the putative active site in the C-terminal region is thought to be responsible for the generation of maximal proteolytic function in the pH range 8.5-10.7 and enhanced stability to denaturation.


===THE PRIMARY STRUCTURE AND STRUCTURAL CHARACTERISTICS OF ACHROMOBACTER LYTICUS PROTEASE I, A LYSINE-SPECIFIC SERINE PROTEASE===
The primary structure and structural characteristics of Achromobacter lyticus protease I, a lysine-specific serine protease.,Tsunasawa S, Masaki T, Hirose M, Soejima M, Sakiyama F J Biol Chem. 1989 Mar 5;264(7):3832-9. PMID:2492988<ref>PMID:2492988</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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The line below this paragraph, {{ABSTRACT_PUBMED_2492988}}, adds the Publication Abstract to the page
<div class="pdbe-citations 1arb" style="background-color:#fffaf0;"></div>
(as it appears on PubMed at http://www.pubmed.gov), where 2492988 is the PubMed ID number.
== References ==
-->
<references/>
{{ABSTRACT_PUBMED_2492988}}
__TOC__
 
</StructureSection>
==About this Structure==
1ARB is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Achromobacter_lyticus Achromobacter lyticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ARB OCA].
 
==Reference==
The primary structure and structural characteristics of Achromobacter lyticus protease I, a lysine-specific serine protease., Tsunasawa S, Masaki T, Hirose M, Soejima M, Sakiyama F, J Biol Chem. 1989 Mar 5;264(7):3832-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/2492988 2492988]
[[Category: Achromobacter lyticus]]
[[Category: Achromobacter lyticus]]
[[Category: Lysyl endopeptidase]]
[[Category: Large Structures]]
[[Category: Single protein]]
[[Category: Katsube Y]]
[[Category: Katsube, Y.]]
[[Category: Kitagawa Y]]
[[Category: Kitagawa, Y.]]
 
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Retrieved from "https://proteopedia.org/1arb"